Optimization of a preparative capillary gas chromatography-mass spectrometry system for the isolation and harvesting of individual polycyclic aromatic hydrocarbons

被引：42

作者：

Mandalakis, M ^{[1
]}

Gustafsson, Ö ^{[1
]}

机构：

[1] Stockholm Univ, ITM, Inst Appl Environm Res, S-10691 Stockholm, Sweden

来源：

JOURNAL OF CHROMATOGRAPHY A | 2003年 / 996卷 / 1-2期

关键词：

preparative chromatography; instrumentation; injection methods; polynuclear aromatic hydrocarbons;

D O I：

10.1016/S0021-9673(03)00612-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Operation parameters of a preparative capillary gas chromatography (pcGC) system were optimized to facilitate clean and efficient harvesting of individual polycyclic aromatic hydrocarbons (PAHs) for subsequent compound-specific radiocarbon analysis. For PAHs, the recommended optimized settings of the specially-designed pcGC cooled injection system (CIS) and preparative fraction collector (PFC) are: 5 s CIS solvent venting time, deactivation of CIS "stop flow" injection mode, autoinjector "fast injection" mode, 60 s CIS splitless time, 340 degreesC PFC switch temperature, and 30 degreesC (ambient) trapping temperature. These optimized conditions yielded highly reproducible, pure, and efficient pcGC harvesting of six PAHs with mass recoveries of 90-100% and purity of the isolates of 97-100%. (C) 2003 Elsevier Science B.V. All rights reserved.

引用

页码：163 / 172

页数：10

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