Intermediates of the Autocatalytic Reaction of the Formation of a Chromophore in a Green Fluorescent Protein

被引：5
|
作者：
Grigorenko, B. L. [1 ,2 ]
Khrenova, M. G. [2 ,3 ]
Kulakova, A. M. [2 ]
Nemukhin, A. V. [1 ,2 ]
机构：
[1] Russian Acad Sci, Emanuel Inst Biochem Phys, Moscow 119991, Russia
[2] Moscow MV Lomonosov State Univ, Moscow 119234, Russia
[3] Russian Acad Sci, Bach Inst Biochem, Fed Res Ctr Fundamentals Biotechnol, Moscow 119071, Russia
来源：
RUSSIAN JOURNAL OF PHYSICAL CHEMISTRY B | 2020年 / 14卷 / 03期
基金：
俄罗斯基础研究基金会;
关键词：
intermediates of enzymatic reactions; green fluorescent protein; autocatalytic chromophore formation;
D O I：
10.1134/S1990793120030161
中图分类号：
O64 [物理化学（理论化学）、化学物理学]; O56 [分子物理学、原子物理学];
学科分类号：
070203 ; 070304 ; 081704 ; 1406 ;
摘要：
The autocatalytic formation of a chromophore in a green fluorescent protein, which is actively used as a marker for visualizing processes in living systems, is an important example of multistage reactions in protein macromolecules. The kinetic, spectral, and X-ray structural experimental studies and supercomputer calculations by molecular modeling methods are devoted to the study of intermediates of the elementary reaction stages, which are grouped into cyclization and oxidation blocks. The article analyzes the results, compatible with the experimental data, of the structure of intermediates of the reactions of chromophore formation for the native protein form obtained through computer modeling, with the aim of including this information in the database of the reactions' intermediates in enzymatic reactions.
引用
收藏
页码：457 / 461
页数：5
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