Adaptive optics correction of specimen-induced aberrations in single-molecule switching microscopy

被引：88

作者：

Burke, Daniel ^{[1
]}

Patton, Brian ^{[1
]}

Huang, Fang ^{[3
]}

Bewersdorf, Joerg ^{[3
,4
]}

Booth, Martin J. ^{[1
,2
]}

机构：

[1] Univ Oxford, Ctr Neural Circuits & Behav, Oxford OX1 3SR, England

[2] Univ Oxford, Dept Engn Sci, Oxford OX1 3PJ, England

[3] Yale Univ, Dept Cell Biol, Sch Med, New Haven, CT 06511 USA

[4] Yale Univ, Dept Biomed Engn, New Haven, CT 06511 USA

来源：

OPTICA | 2015年 / 2卷 / 02期

基金：

英国惠康基金;

关键词：

SUPERRESOLUTION MICROSCOPY; RECONSTRUCTION MICROSCOPY; LOCALIZATION; RESOLUTION; NANOSCOPY; TRACKING; IMAGE;

D O I：

10.1364/OPTICA.2.000177

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

Single-molecule switching (SMS) microscopy is a super-resolution method capable of producing images with resolutions far exceeding that of the classical diffraction limit. However, like all optical microscopes, SMS microscopes are sensitive to, and often limited by, specimen-induced aberrations. Adaptive optics (AO) has proven beneficial in a range of microscopes to overcome the limitations caused by aberrations. We report here on new AO methods for SMS microscopy that enable the feedback correction of specimeninduced aberrations. The benefits are demonstrated through two-dimensional and three-dimensional STORM imaging. We expect that this advance will broaden the scope of SMS microscopy by enabling deep-cell and tissue-level imaging. (C) 2015 Optical Society of America

引用

页码：177 / 185

页数：9

共 35 条

[1]

Abbe E., 1873, Archiv f ur Mikroskopische Anatomie, V9, P413, DOI DOI 10.1007/BF02956173

[2] Imaging intracellular fluorescent proteins at nanometer resolution [J].

Betzig, Eric ;

Patterson, George H. ;

Sougrat, Rachid ;

Lindwasser, O. Wolf ;

Olenych, Scott ;

Bonifacino, Juan S. ;

Davidson, Michael W. ;

Lippincott-Schwartz, Jennifer ;

Hess, Harald F. .

SCIENCE, 2006, 313 (5793) :1642-1645

[3] Adaptive optical microscopy: the ongoing quest for a perfect image [J].

Booth, Martin J. .

LIGHT-SCIENCE & APPLICATIONS, 2014, 3 :e165-e165

[4] Aberration correction for confocal imaging in refractive-index-mismatched media [J].

Booth, MJ ;

Neil, MAA ;

Wilson, T .

JOURNAL OF MICROSCOPY, 1998, 192 :90-98

[5] Adaptive aberration correction in a confocal microscope [J].

Booth, MJ ;

Neil, MAA ;

Juskaitis, R ;

Wilson, T .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (09) :5788-5792

[6] Simple characterisation of a deformable mirror inside a high numerical aperture microscope using phase diversity [J].

Debarre, D. ;

Vieille, T. ;

Beaurepaire, E. .

JOURNAL OF MICROSCOPY, 2011, 244 (02) :136-143

[7] Achieving increased resolution and more pixels with Superresolution Optical Fluctuation Imaging (SOFI) [J].

Dertinger, Thomas ;

Colyer, Ryan ;

Vogel, Robert ;

Enderlein, Joerg ;

Weiss, Shimon .

OPTICS EXPRESS, 2010, 18 (18) :18875-18885

[8] Molecular Orientation Affects Localization Accuracy in Superresolution Far-Field Fluorescence Microscopy [J].

Engelhardt, Johann ;

Keller, Jan ;

Hoyer, Patrick ;

Reuss, Matthias ;

Staudt, Thorsten ;

Hell, Stefan W. .

NANO LETTERS, 2011, 11 (01) :209-213

[9] Adaptive optics enables 3D STED microscopy in aberrating specimens [J].

Gould, Travis J. ;

Burke, Daniel ;

Bewersdorf, Joerg ;

Booth, Martin J. .

OPTICS EXPRESS, 2012, 20 (19) :20998-21009

[10]

Gould TJ, 2012, ANNU REV BIOMED ENG, V14, P231, DOI [10.1146/annurev-bioeng-071811-150025, 10.1146/annurev.bioeng-071811-150025]

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