Isolation of microsatellite markers from an interspecific hybrid isolate of the fungal plant pathogen Verticillium dahliae

被引：5

作者：

Barbara, DJ ^{[1
]}

Morton, A ^{[1
]}

Miller, NJ ^{[1
]}

机构：

[1] Warwick HRI, Wellesbourne CV35 9EF, Warwick, England

来源：

MOLECULAR ECOLOGY NOTES | 2005年 / 5卷 / 04期

关键词：

interspecific hybrids; microsatellite; vascular wilts; Verticillium albo-atrum; Verticillium dahliae;

D O I：

10.1111/j.1471-8286.2005.01087.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Ten microsatellite markers have been isolated from an amphihaploid isolate of Verticillium dahliae using genomic libraries enriched for (AGT), (GAC), (GCC), (TAC) and (TTA) repetitive elements. These were characterized on four amphihaploid isolates and two haploid isolates each of V. dahliae and Verticillium albo-atrum. Nine were polymorphic, with between two and five alleles, and the tenth was polymorphic when tested on a further 20 haploid V. dahliae isolates (three alleles). Only one primer pair gave the expected double bands from the amphihaploid isolates, supporting the view that V. albo-atrum is not one of the parents of the interspecific hybrid amphihaploid isolates.

引用

页码：854 / 856

页数：3

共 5 条

[1] Barbara DJ, 2003, MOL PLANT PATHOL, V4, P297, DOI [10.1046/j.1364-3703.2003.00172.x, 10.1046/J.1364-3703.2003.00172.X]
[2] Isolates of Verticillium dahliae pathogenic to crucifers are of at least three distinct molecular types
Collins, A
Okoli, CAN
Morton, A
Parry, D
Edwards, SG
Barbara, DJ
[J]. PHYTOPATHOLOGY, 2003, 93 (03) : 364 - 376
[3] Edwards KJ, 1996, BIOTECHNIQUES, V20, P758
[4] Hodge KT, 2005, TAXON, V54, P485, DOI 10.2307/25065318
[5] Morphological and molecular characterization of Verticillium longisporum comb. nov., pathogenic to oilseed rape
Karapapa, VK
Bainbridge, BW
Heale, JB
[J]. MYCOLOGICAL RESEARCH, 1997, 101 : 1281 - 1294

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