Effects of ampicillin on cystine/glutamate antiporter and glutamate transporter 1 isoforms as well as ethanol drinking in male P rats

被引:23
作者
Alasmari, Fawaz [1 ]
Abuhamdah, Sawsan [1 ]
Sari, Youssef [1 ]
机构
[1] Univ Toledo, Coll Pharm & Pharmaceut Sci, Dept Pharmacol & Expt Therapeut, Hlth Sci Campus,3000 Arlington Ave, Toledo, OH 43614 USA
关键词
Ampicillin; Ethanol intake; Glutamate GLT-1a; GLT-1b; xCT; GLAST; ALCOHOL-PREFERRING RATS; GLT1; MODULATION; CEFTRIAXONE; CONSUMPTION; NEURONS; BRAIN; NEUROPROTECTION; EXPRESSION; ADDICTION; BEHAVIOR;
D O I
10.1016/j.neulet.2015.06.015
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Evidence demonstrated that glial cells, mainly astrocytes, regulate glutamate uptake through several glutamate transporters. Among these glutamate transporters, glutamate transporter 1 (GLT-1; its human homolog is excitatory amino acid transporter-2) is responsible for the majority of glutamate uptake. Cystine-glutamate antiporter (xCT) is another glial protein critical in regulating glutamate transmission. Several studies from our laboratory demonstrated that attenuation of ethanol intkae was associated in part with upregulation of xCT and GLT-1 expression suggesting the important role of these transporters in the treatment of ethanol dependence. We found recently that beta-lactam antibiotic, ampicillin, upregulated GLT-1 expression in the prefrontal cortex (PFC) and nucleus accumbens (NAc) and consequently reduced ethanol intake in alcohol-preferring (P) rats. In this study, we investigated the effects of ampicillin on the expression of xCT and GLT-1 isoforms (GLT-1a and GLT-1b) as well as on GLAST expression. We found that ampicillin reduced ethanol intake as compared to the saline (control)-treated group. In addition, we found that ampicillin induced upregulation of xCT, GLT-1a, and GLT-1b expression in both the PFC and NAc, but had no effect on GLAST expression. Our findings provide significant role of ampicillin on upregulating xCT and GLT-1 isoforms expression, might be suggested as possible targets for the attenuation of ethanol consumption. (C) 2015 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码:148 / 152
页数:5
相关论文
共 28 条
  • [1] Effects of MS-153 on chronic ethanol consumption and GLT1 modulation of glutamate levels in male alcohol-preferring rats
    Alhaddad, Hasan
    Kim, Nathaniel T.
    Aal-Aaboda, Munaf
    Althobaiti, Yusuf S.
    Leighton, James
    Boddu, Sai H. S.
    Wei, Yangjie
    Sari, Youssef
    [J]. FRONTIERS IN BEHAVIORAL NEUROSCIENCE, 2014, 8
  • [2] Effects of ceftriaxone on ethanol intake: a possible role for xCT and GLT-1 isoforms modulation of glutamate levels in P rats
    Alhaddad, Hasan
    Das, Sujan C.
    Sari, Youssef
    [J]. PSYCHOPHARMACOLOGY, 2014, 231 (20) : 4049 - 4057
  • [3] Cellular and subcellular mRNA localization of glutamate transporter isoforms GLT1a and GLT1b in rat brain by in situ hybridization
    Berger, UV
    DeSilva, TM
    Chen, WZ
    Rosenberg, PA
    [J]. JOURNAL OF COMPARATIVE NEUROLOGY, 2005, 492 (01) : 78 - 89
  • [4] The glutamate transporter GLT1a is expressed in excitatory axon terminals of mature hippocampal neurons
    Chen, WZ
    Mahadomrongkul, V
    Berger, UV
    Bassan, M
    DeSilva, T
    Tanaka, K
    Irwin, N
    Aoki, C
    Rosenberg, PA
    [J]. JOURNAL OF NEUROSCIENCE, 2004, 24 (05) : 1136 - 1148
  • [5] Expression of a variant form of the glutamate transporter GLT1 in neuronal cultures and in neurons and astrocytes in the rat brain
    Chen, WZ
    Aoki, C
    Mahadomrongkul, V
    Gruber, CE
    Wang, GJ
    Blitzblau, R
    Irwin, N
    Rosenberg, PA
    [J]. JOURNAL OF NEUROSCIENCE, 2002, 22 (06) : 2142 - 2152
  • [6] Glutamate uptake
    Danbolt, NC
    [J]. PROGRESS IN NEUROBIOLOGY, 2001, 65 (01) : 1 - 105
  • [7] Ethanol Increases Glutamate Neurotransmission in the Posterior Ventral Tegmental Area of Female Wistar Rats
    Ding, Zheng-Ming
    Engleman, Eric A.
    Rodd, Zachary A.
    McBride, William J.
    [J]. ALCOHOLISM-CLINICAL AND EXPERIMENTAL RESEARCH, 2012, 36 (04) : 633 - 640
  • [8] Pharmacogenetic approaches to the treatment of alcohol addiction
    Heilig, Markus
    Goldman, David
    Berrettini, Wade
    O'Brien, Charles P.
    [J]. NATURE REVIEWS NEUROSCIENCE, 2011, 12 (11) : 670 - 684
  • [9] THE CONCENTRATIONS AND DISTRIBUTIONS OF THREE C-TERMINAL VARIANTS OF THE GLT1 (EAAT2; slc1a2) GLUTAMATE TRANSPORTER PROTEIN IN RAT BRAIN TISSUE SUGGEST DIFFERENTIAL REGULATION
    Holmseth, S.
    Scott, H. A.
    Real, K.
    Lehre, K. P.
    Leergaard, T. B.
    Bjaalie, J. G.
    Danbolt, N. C.
    [J]. NEUROSCIENCE, 2009, 162 (04) : 1055 - 1071
  • [10] Excitatory amino acid transporters: recent insights into molecular mechanisms, novel modes of modulation and new therapeutic possibilities
    Jensen, Anders A.
    Fahlke, Christoph
    Bjorn-Yoshimoto, Walden E.
    Bunch, Lennart
    [J]. CURRENT OPINION IN PHARMACOLOGY, 2015, 20 : 116 - 123