Thyroid hormone promotes cell invasion through activation of furin expression in human hepatoma cell lines

被引：50

作者：

Chen, Ruey-Nan ^{[1
]}

Huang, Ya-Hui ^{[1
]}

Lin, Ya-Chu ^{[1
]}

Yeh, Chau-Ting ^{[2
,5
]}

Liang, Ying ^{[3
]}

Chen, Shen-Liang ^{[4
]}

Lin, Kwang-Huei ^{[1
]}

机构：

[1] Chang Gung Univ, Dept Biochem, Tao Yuan 333, Taiwan

[2] Chang Gung Univ, Dept Med, Tao Yuan 333, Taiwan

[3] Chang Gung Univ, Sch Med, Proteom Core Lab, Tao Yuan 333, Taiwan

[4] Natl Cent Univ, Dept Life Sci, Jhongli 320, Taiwan

[5] Chang Gung Med Ctr, Liver Res Unit, Taipei 10591, Taiwan

来源：

ENDOCRINOLOGY | 2008年 / 149卷 / 08期

关键词：

D O I：

10.1210/en.2007-0989

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

The objective of this study was to identify genes regulated by thyroid hormone (T-3) and associated with tumor invasion. The gene encoding furin, as previously identified by cDNA microarray, is known to be up-regulated by T-3 treatment, and stimulated furin production occurs in thyroidectomized rats after administration of T-3. Presently, by using serial deletion of the promoter and EMSAs, the T-3 response element on the furin promoter was localized to the -6317/-6302 region. T-3-mediated furin up-regulation was cooperative with TGF-beta because T-3 induction increased after Smad3/4 addition. Furthermore, the invasiveness of HepG2-thyroid hormone receptor (TR) cells was significantly increased by T-3 treatment, perhaps due to furin processing of matrix metalloproteinase-2 and -9. In addition, furin up-regulation either by stable overexpression

引用

页码：3817 / 3831

页数：15

共 54 条

[11] TGF beta 1 regulates gene expression of its own converting enzyme furin
Blanchette, F
Day, R
Dong, W
Laprise, MH
Dubois, CM
[J]. JOURNAL OF CLINICAL INVESTIGATION, 1997, 99 (08) : 1974 - 1983
[12] Involvement of Smads in TGFβ1-induced furin (fur) transcription
Blanchette, F
Rudd, P
Grondin, F
Attisano, L
Dubois, CM
[J]. JOURNAL OF CELLULAR PHYSIOLOGY, 2001, 188 (02) : 264 - 273
[13] Cross-talk between the p42/p44 MAP kinase and Smad pathways in transforming growth factor β1-induced furin gene transactivation
Blanchette, F
Rivard, N
Rudd, P
Grondin, F
Attisano, L
Dubois, CM
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (36) : 33986 - 33994
[14] Expression and function of thyroid hormone receptor variants in normal and chronically diseased human liver
Chamba, A
Neuberger, J
Strain, A
Hopkins, J
Sheppard, MC
Franklyn, JA
[J]. JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 1996, 81 (01) : 360 - 367
[15] INDUCTION OF PLASMA-PROTEIN SECRETION IN A NEWLY ESTABLISHED HUMAN HEPATOMA-CELL LINE
CHANG, C
LIN, Y
OLEE, TW
CHOU, CK
LEE, TS
LIU, TJ
PENG, FK
CHEN, TY
HU, CP
[J]. MOLECULAR AND CELLULAR BIOLOGY, 1983, 3 (06) : 1133 - 1137
[16] Mitogen-activated protein kinases potentiate thyroid hormone receptor transcriptional activity by stabilizing its protein
Chen, SL
Chang, YJ
Wu, YH
Lin, KH
[J]. ENDOCRINOLOGY, 2003, 144 (04) : 1407 - 1419
[17] Cheng M, 1997, INT J CANCER, V71, P966, DOI 10.1002/(SICI)1097-0215(19970611)71:6<966::AID-IJC10>3.3.CO
[18] 2-A
[19] Multiple Mechanisms for Regulation of the Transcriptional Activity of Thyroid Hormone Receptors
Sheue-yann Cheng
[J]. Reviews in Endocrine and Metabolic Disorders, 2000, 1 (1-2) : 9 - 18
[20] TGF-β induces fibronectin synthesis through a c-Jun N-terminal kinase-dependent, Smad4-independent pathway
Hocevar, BA
Brown, TL
Howe, PH
[J]. EMBO JOURNAL, 1999, 18 (05) : 1345 - 1356

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