Comparative Study of Fourteen Alkaloids from Uncaria rhynchophylla Hooks and Leaves Using HPLC-Diode Array Detection-Atmospheric Pressure Chemical Ionization/MS Method

被引：27
作者：
Qu, Jialin [1 ]
Gong, Tianxing [2 ]
Ma, Bin [1 ]
Zhang, Lin [1 ]
Kano, Yoshihiro [1 ]
Yuan, Dan [1 ]
机构：
[1] Shenyang Pharmaceut Univ, Sch Tradit Chinese Med, Shenyang 110016, Peoples R China
[2] Univ British Columbia, Vancouver, BC V6T 1Z1, Canada
来源：
CHEMICAL & PHARMACEUTICAL BULLETIN | 2012年 / 60卷 / 01期
关键词：
oxindole alkaloid; glycosidic alkaloid; Uncaria rhynchophylla; alkaloid profile; HPLC; LC-atmospheric pressure chemical ionization-MS; INDOLE ALKALOIDS; OXINDOLE ALKALOIDS; ISORHYNCHOPHYLLINE; RATS; METABOLISM; SINENSIS; PLANTS;
D O I：
10.1248/cpb.60.23
中图分类号：
R914 [药物化学];
学科分类号：
100701 ;
摘要：
The purpose of the study is to compare alkaloid profile of Uncaria rhynchophylla hooks and leaves. Ten oxindole alkaloids and four glycosidic indole alkaloids were identified using HPLC-diode array detection (DAD) or LC-atmospheric pressure chemical ionization (APCI)-MS method, and a HPLC-UV method for simultaneous quantification of major alkaloids was validated. The hooks are characterized by high levels of four oxindole alkaloids rhynchophylline (R), isorhynchophylline (IR), corynoxeine (C) and isocorynoxeine (IC), while the leaves contained high level of two glycosidic indole alkaloids vincoside lactam (VL) and strictosidine (S). The presented methods have proven its usefulness in chemical characterization of U. rhynchophylla hooks and leaves.
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页码：23 / 30
页数：8
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