Comparative Study of Fourteen Alkaloids from Uncaria rhynchophylla Hooks and Leaves Using HPLC-Diode Array Detection-Atmospheric Pressure Chemical Ionization/MS Method

被引：30

作者：

Qu, Jialin ^{[1
]}

Gong, Tianxing ^{[2
]}

Ma, Bin ^{[1
]}

Zhang, Lin ^{[1
]}

Kano, Yoshihiro ^{[1
]}

Yuan, Dan ^{[1
]}

机构：

[1] Shenyang Pharmaceut Univ, Sch Tradit Chinese Med, Shenyang 110016, Peoples R China

[2] Univ British Columbia, Vancouver, BC V6T 1Z1, Canada

来源：

CHEMICAL & PHARMACEUTICAL BULLETIN | 2012年 / 60卷 / 01期

关键词：

oxindole alkaloid; glycosidic alkaloid; Uncaria rhynchophylla; alkaloid profile; HPLC; LC-atmospheric pressure chemical ionization-MS; INDOLE ALKALOIDS; OXINDOLE ALKALOIDS; ISORHYNCHOPHYLLINE; RATS; METABOLISM; SINENSIS; PLANTS;

D O I：

10.1248/cpb.60.23

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

The purpose of the study is to compare alkaloid profile of Uncaria rhynchophylla hooks and leaves. Ten oxindole alkaloids and four glycosidic indole alkaloids were identified using HPLC-diode array detection (DAD) or LC-atmospheric pressure chemical ionization (APCI)-MS method, and a HPLC-UV method for simultaneous quantification of major alkaloids was validated. The hooks are characterized by high levels of four oxindole alkaloids rhynchophylline (R), isorhynchophylline (IR), corynoxeine (C) and isocorynoxeine (IC), while the leaves contained high level of two glycosidic indole alkaloids vincoside lactam (VL) and strictosidine (S). The presented methods have proven its usefulness in chemical characterization of U. rhynchophylla hooks and leaves.

引用

页码：23 / 30

页数：8

共 24 条

[1]

[Anonymous], J HIGH RESOL CHROMAT

[2]

[Anonymous], J TRADIT MED

[3]

Bensky D., 1999, Chinese Medicine: Materia Medica, P423

[4] Protective effect of rhynchophylline and isorhynchophylline on in vitro ischemia-induced neuronal damage in the hippocampus: putative neurotransmitter receptors involved in their action [J].

Kang, TH ;

Murakami, Y ;

Takayama, H ;

Kitajima, M ;

Aimi, N ;

Watanabe, H ;

Matsumoto, K .

LIFE SCIENCES, 2004, 76 (03) :331-343

[5] Kinetics of isomerization of tetracyclic spiro oxindole alkaloids [J].

Laus, G .

JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 2, 1998, (02) :315-317

[6] OXINDOLE ALKALOIDS FROM UNCARIA-SINENSIS [J].

LIU, HM ;

FENG, XZ .

PHYTOCHEMISTRY, 1993, 33 (03) :707-710

[7] Three New Alkaloids from the Leaves of Uncaria rhynchophylla [J].

Ma, Bin ;

Wu, Chun-Fu ;

Yang, Jing-Yu ;

Wang, Rui ;

Kano, Yoshihiro ;

Yuan, Dan .

HELVETICA CHIMICA ACTA, 2009, 92 (08) :1575-1585

[8] Identification and quantification of components in extracts of Uncaria tomentosa by HPLC-ES/MS [J].

Montoro, P ;

Carbone, V ;

Quiroz, JD ;

De Simone, F ;

Pizza, C .

PHYTOCHEMICAL ANALYSIS, 2004, 15 (01) :55-64

[9]

PHILLIPSON JD, 1978, LLOYDIA, V41, P503

[10] Effect on locomotion of indole alkaloids from the hooks of Uncaria plants [J].

Sakakibara, I ;

Terabayashi, S ;

Kubo, M ;

Higuchi, M ;

Komatsu, Y ;

Okada, M ;

Taki, K ;

Kamei, J .

PHYTOMEDICINE, 1999, 6 (03) :163-168

← 1 2 3 →