Electrospray ionization and matrix assisted laser desorption/ionization mass spectrometry: Powerful analytical tools in recombinant protein chemistry

被引：95
作者：
Andersen, JS
Svensson, B
Roepstorff, P
机构：
[1] ODENSE UNIV, DEPT MOLEC BIOL, DK-5230 ODENSE M, DENMARK
[2] CARLSBERG LAB, DEPT CHEM, DK-2500 COPENHAGEN, DENMARK
来源：
NATURE BIOTECHNOLOGY | 1996年 / 14卷 / 04期
关键词：
D O I：
10.1038/nbt0496-449
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Electrospray ionization and matrix assisted laser desorption/ionization are effective ionization methods for mass spectrometry of biomolecules. Here we describe the capabilities of these methods for peptide and protein characterization in biotechnology. An integrated analytical strategy is presented encompassing protein characterization prior to and after cloning of the corresponding gene.
引用
收藏
页码：449 / 457
页数：9
相关论文
共 112 条
[1]  
Aebersol Ruedi, 1993, Current Opinion in Biotechnology, V4, P412, DOI 10.1016/0958-1669(93)90006-I
[2]  
AMANKWA LN, 1995, PROTEIN SCI, V4, P113
[3]   LOCALIZATION OF AN O-GLYCOSYLATED SITE IN THE RECOMBINANT BARLEY ALPHA-AMYLASE-1 PRODUCED IN YEAST AND CORRECTION OF THE AMINO-ACID-SEQUENCE USING MATRIX-ASSISTED LASER-DESORPTION IONIZATION MASS-SPECTROMETRY OF PEPTIDE MIXTURES [J].
ANDERSEN, JS ;
SOGAARD, M ;
SVENSSON, B ;
ROEPSTORFF, P .
BIOLOGICAL MASS SPECTROMETRY, 1994, 23 (09) :547-554
[4]   PURITY ANALYSIS OF PROTEIN PHARMACEUTICALS PRODUCED BY RECOMBINANT DNA TECHNOLOGY [J].
ANICETTI, VR ;
KEYT, BA ;
HANCOCK, WS .
TRENDS IN BIOTECHNOLOGY, 1989, 7 (12) :342-349
[5]   MATRIX-ASSISTED LASER DESORPTION USING A FAST-ATOM-BOMBARDMENT ION-SOURCE AND A MAGNETIC MASS-SPECTROMETER [J].
ANNAN, RS ;
KOCHLING, HJ ;
HILL, JA ;
BIEMANN, K .
RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 1992, 6 (04) :298-302
[6]   ULTRASONICALLY ASSISTED ELECTROSPRAY-IONIZATION FOR LC/MS DETERMINATION OF NUCLEOSIDES FROM A TRANSFER-RNA DIGEST [J].
BANKS, JF ;
SHEN, S ;
WHITEHOUSE, CM ;
FENN, JB .
ANALYTICAL CHEMISTRY, 1994, 66 (03) :406-414
[7]  
BARBER M, 1982, ANAL CHEM, V54, pA645
[8]   CHARACTERIZATION OF DISULFIDE BOND POSITION IN PROTEINS AND SEQUENCE-ANALYSIS OF CYSTINE-BRIDGED PEPTIDES BY TANDEM MASS-SPECTROMETRY [J].
BEAN, MF ;
CARR, SA .
ANALYTICAL BIOCHEMISTRY, 1992, 201 (02) :216-226
[9]  
Beavis R C, 1989, Rapid Commun Mass Spectrom, V3, P432, DOI 10.1002/rcm.1290031207
[10]   HIGH-ACCURACY MOLECULAR MASS DETERMINATION OF PROTEINS USING MATRIX-ASSISTED LASER DESORPTION MASS-SPECTROMETRY [J].
BEAVIS, RC ;
CHAIT, BT .
ANALYTICAL CHEMISTRY, 1990, 62 (17) :1836-1840
12345678910