Extracellular homopolysaccharides and oligosaccharides from intestinal lactobacilli

被引:43
作者
Tieking, M
Kaditzky, S
Valcheva, R
Korakli, M
Vogel, RF
Gänzle, MG
机构
[1] Tech Univ Munich, Lehrstuhl Tech Mikrobiol, D-8050 Freising Weihenstephan, Germany
[2] Lab Microbiol Alimentaire & Ind Enitiaa, Nantes, France
关键词
extracellular polysaccharides; fructan; fructose-oligosaccharides; glucan; glucose-oligosaccharides; intestinal lactobacilli; Lactobacillus reuteri;
D O I
10.1111/j.1365-2672.2005.02638.x
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Aims: To characterize lactobacilli isolated from the intestines of ducks or pigs with respect to the production of extracellular homopolysaccharides (HoPS) and oligosaccharides. Methods and Results: Lactobacillus strains of duck or pig origin were screened for HoPS synthesis and > 25% of the isolates produced fructans or glucans from sucrose. Glucan-forming strains were found within the species Lactobacillus reuteri and Lactobacillus animalis and fructan-forming strains were found within Lactobacillus mucosae, Lactobacillus crispatus and Lactobacillus acidophilus. The glucan-forming strains of L. reuteri but not L. animalis produced glucose-oligosaccharides in additon to the respective polymers, and two fructan-forming strains of L. acidophilus produced kestose. Genes coding for glycosyltransferases were detected by PCR and partially characterized by sequence analysis. Conclusions: A large proportion of lactobacilli from intestinal habitats produce HoPS from sucrose and polysaccharide formation is generally associated with the formation of glucose- and fructose oligosaccharides. Significance and Impact of the Study: The characterization of the metabolic potential of intestinal lactobacilli contributes to the understanding of the molecular basis of autochthony in intestinal habitats. Moreover, this is the first report of glucose-oligosaccharide production during growth of lactobacilli, and one novel fructosyltransferase and one novel glucansucrase were partially characterized on the genetic level.
引用
收藏
页码:692 / 702
页数:11
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