Proteomic analysis of the outer membrane of Anabaena sp strain PCC 7120

被引:52
作者
Moslavac, S [1 ]
Bredemeier, R [1 ]
Mirus, O [1 ]
Granvogl, B [1 ]
Eichacker, LA [1 ]
Schleiff, E [1 ]
机构
[1] Univ Munich, Dept Biol, D-80638 Munich, Germany
关键词
proteome analysis; beta-barrel analysis; Anabaena; outer membrane;
D O I
10.1021/pr050044c
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Anabaena is a model to analyze the evolutionary development of plastids, cell differentiation, and the regulation of nitrogen fixation. Thereby, the outer membrane proteome is the place of sensing environmental differences and during plastid development, systems for intracellular communication had to be added to the proteome of this membrane. We present a protocol for the isolation of the outer membrane from Anabaena and the analysis of the proteome using different tools. 55 Proteins were identified.
引用
收藏
页码:1330 / 1338
页数:9
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