Collagen Type I and II Blend Hydrogel with Autologous Mesenchymal Stem Cells as a Scaffold for Articular Cartilage Defect Repair

被引:83
作者
Kilmer, Claire [1 ]
Battistoni, Carly M. [1 ]
Cox, Abigail [3 ]
Breur, Gert J. [4 ]
Panitch, Alyssa [2 ,5 ]
Liu, Julie C. [1 ,2 ]
机构
[1] Purdue Univ, Davidson Sch Chem Engn, W Lafayette, IN 47907 USA
[2] Purdue Univ, Weldon Sch Biomed Engn, W Lafayette, IN 47907 USA
[3] Purdue Univ, Dept Comparat Pathobiol, W Lafayette, IN 47907 USA
[4] Purdue Univ, Dept Vet Clin Sci, W Lafayette, IN 47907 USA
[5] Univ Calif Davis, Sch Biomed Engn, Davis, CA 95616 USA
基金
美国国家卫生研究院;
关键词
extracellular matrix; fibrillogenesis; osteoarthritis; rabbit model; chondrogenic differentiation; FULL-THICKNESS DEFECTS; CHONDROGENIC DIFFERENTIATION; EXTRACELLULAR-MATRIX; OSTEOCHONDRAL DEFECTS; SURGICAL-TREATMENT; BASIC SCIENCE; CROSS-LINKING; BONE; CHONDROCYTES; TISSUE;
D O I
10.1021/acsbiomaterials.9b01939
中图分类号
TB3 [工程材料学]; R318.08 [生物材料学];
学科分类号
0805 ; 080501 ; 080502 ;
摘要
Collagen type II is a promising material to repair cartilage defects since it is a major component of articular cartilage and plays a key role in chondrocyte function. This study investigated the chondrogenic differentiation of bone marrow-derived mesenchymal stem cells (MSCs) embedded within a 3:1 collagen type I to II blend (Col I/II) hydrogel or an all collagen type I (Col I) hydrogel. Glycosaminoglycan (GAG) production in Col I/II hydrogels was statistically higher than that in Col I hydrogels or pellet culture, and these results suggested that adding collagen type II promoted GAG production. Col I/II hydrogels had statistically lower alkaline phosphatase (AP) activity than pellets cultured in a chondrogenic medium. The ability of MSCs encapsulated in Col I/II hydrogels to repair cartilage defects was investigated by creating two cartilage defects in the femurs of rabbits. After 13 weeks, histochemical staining suggested that Col I/II blend hydrogels provided favorable conditions for cartilage repair. Histological scoring revealed a statistically higher cartilage repair score for the Col I/II hydrogels compared to either the Col I hydrogels or empty defect controls. Results from this study suggest that there is clinical value in the cartilage repair capabilities of our Col I/II hydrogel with encapsulated MSCs.
引用
收藏
页码:3464 / 3476
页数:13
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