Antagonistic Effect of Short Neuropeptide F on Allatotropin-Inhibited Feeding Motivation of the Silkworm Larva, Bombyx mori

被引：4

作者：

Matsumoto, Sumihiro ^{[1
]}

Ohara, Ayako ^{[2
]}

Nagai-Okatani, Chiaki ^{[2
,3
]}

Zhou, Yi-Jun ^{[1
,4
]}

Fujinaga, Daiki ^{[1
]}

Seike, Hitomi ^{[1
]}

Nagata, Shinji ^{[1
]}

机构：

[1] Univ Tokyo, Grad Sch Frontier Sci, Dept Integrated Biosci, 5-1-5 Kashiwanoha, Kashiwa, Chiba 2778562, Japan

[2] Univ Tokyo, Grad Sch Agr & Life Sci, Dept Appl Biol Chem, Bunkyo Ku, 1-1-1 Yayoi, Tokyo 1138657, Japan

[3] Natl Inst Adv Ind Sci & Technol, Biotechnol Res Inst Drug Discovery, Tsukuba, Ibaraki 3058568, Japan

[4] JSPS, Chiyoda Ku, Tokyo 1020083, Japan

来源：

ZOOLOGICAL SCIENCE | 2019年 / 36卷 / 01期

基金：

日本学术振兴会;

关键词：

Bombyx mori; short neuropeptide F; allatotropin; antagonistic effect; feeding motivation; BNGRs; JUVENILE-HORMONE BIOSYNTHESIS; MANDUCA-SEXTA ALLATOTROPIN; CORPORA ALLATA; ION-TRANSPORT; PEPTIDES; BEHAVIOR; MOTH; IDENTIFICATION; ALLATOSTATINS; INITIATION;

D O I：

10.2108/zs180119

中图分类号：

Q95 [动物学];

学科分类号：

071002 ;

摘要：

Here, we demonstrated an antagonistic effect of short neuropeptide F (sNPF) in modulating feeding motivation in the silkworm Bombyx mori; sNPF reduced the feeding-delaying effects caused by administration of an inhibitory peptide, allatotropin (AT). In situ hybridization and MALDI-TOF MS analysis revealed the presence of three subtypes of sNPFs (sNPF-1, -2, and -3) in the midgut entero-endocrine cells. Ca2+-imaging analyses revealed that three subtypes of sNPF receptors (sNPFRs) (BNGR-A7, -A10, and -A11) showed different affinities with the three subtypes of sNPFs. In addition, sNPF activated its signaling via ERK phosphorylation in the midgut, while mixture of sNPF and AT reduced the phosphorylation level, agreeing with the results of behavioral assay. Together, our current findings suggest that intestinal sNPF positively modulates the feeding motivation by reducing the inhibitory effects by AT within the midgut.

引用

页码：58 / 67

页数：10