Amycolatopsis alkalitolerans sp. nov., isolated from Gastrodia elata Blume

A Gram-staining positive and nonmotile strain designated SYSUP0005(T) was isolated from tubers of Gastrodia elata Blume. The 16S rRNA gene sequence result showed that strain SYSUP0005(T) shared highest sequence similarity with the type strain of Amycolatopsis cappadoca (95.7%), Amycolatopsis taiwanensis (95.4%), Amycolatopsis pigmentata (95.4%), Amycolatopsis ruanii (95.1%), and Amycolatopsis helveola (94.8%). Growth occurs at 14-37 degrees C (optimum temperature, 28 degrees C), at pH 6-9 (optimum, pH 8) and in the presence of up to 6% (w/v) NaCl. Strain SYSUP0005(T) had meso-diaminopimelic acid in its peptidoglycan. The whole cell sugars were galactose, ribose, and xylose. The predominant menaquinone was MK-9(H-4) and minor menaquinones were MK-9(H-2) and MK-9(H-8). The polar lipids were diphosphatidylglycerol (DPG); phosphatidylmonomethylethanolamine (PME), phosphatidylethanolamine (PE), phosphatidylinositol (PI), unidentified glycolipid (GL), and unidentified phospholipid (PL). The genomic DNA G + C content was 69.6 mol%. The major fatty acids were iso-C-16:0, anteiso-C-17:0, C-16:0, iso-C-14:0, C-17:1 omega 6c, C-17:0, and Summed Feature 3 (C-16:1 omega 7c/C-16:1 omega 6c). On the basis of the phenotypic, phylogenetic, chemotaxonomic characters, and genomic comparison, SYSUP0005(T) represents a novel species of the genus Amycolatopsis, for which the name Amycolatopsis alkalitolerans sp. nov. is proposed. The type strain is SYSUP0005(T) (=KCTC 49024(T) = CGMCC4.7463(T)).