Fluorescence-based detection of single-nucleotide changes in RNA using graphene oxide and DNAzyme

被引：25

作者：

Hong, Chaesun ^{[1
]}

Kim, Dong-Min ^{[1
]}

Baek, Ahruem ^{[1
]}

Chung, Hyewon ^{[2
]}

Jung, Woong ^{[3
]}

Kim, Dong-Eun ^{[1
]}

机构：

[1] Konkuk Univ, Dept Biosci & Biotechnol, Seoul 143701, South Korea

[2] Konkuk Univ, Med Ctr, Dept Ophthalmol, Seoul 134729, South Korea

[3] Kyung Hee Univ Hosp Gangdong, Dept Emergency Med, Seoul 134727, South Korea

来源：

CHEMICAL COMMUNICATIONS | 2015年 / 51卷 / 26期

基金：

新加坡国家研究基金会;

关键词：

CLINICAL RESISTANCE; MASS-SPECTROMETRY; CLEAVAGE; IMATINIB; MUTATION; DNA; DESORPTION; BINDING;

D O I：

10.1039/c4cc09603g

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We report a simple fluorometric method for detection of single-nucleotide changes in RNA using graphene oxide (GO) and RNA-cleaving DNAzyme. The fluorescent DNA probe (F-DNA) was annealed to RNA fragments generated by RNA cleavage with DNAzyme specific to mutant RNA. The F-DNA-RNA duplex attenuated the quenching of F-DNA fluorescence by GO.

引用

页码：5641 / 5644

页数：4

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