Understanding Nucleus Pulposus Cell Phenotype: A Prerequisite for Stem Cell Based Therapies to Treat Intervertebral Disc Degeneration

被引：65
作者：
Choi, Hyowon
Johnson, Zariel I.
Risbud, Makarand V.
机构：
[1] Thomas Jefferson Univ, Dept Orthopaed Surg, Philadelphia, PA 19107 USA
[2] Thomas Jefferson Univ, Grad Program Cell & Dev Biol, Philadelphia, PA 19107 USA
来源：
CURRENT STEM CELL RESEARCH & THERAPY | 2015年 / 10卷 / 04期
关键词：
Annulus fibrosus; cell phenotype; hypoxia; intervertebral disc degeneration; intervertebral disc; nucleus pulposus; stem cell therapy; MESENCHYMAL STROMAL CELLS; NOTOCHORDAL CELLS; PROGENITOR CELLS; IN-VITRO; EXTRACELLULAR-MATRIX; CONDITIONED MEDIUM; ANULUS FIBROSUS; GENE-EXPRESSION; DIFFERENTIATION; HYPOXIA;
D O I：
10.2174/1574888X10666150113112149
中图分类号：
Q813 [细胞工程];
学科分类号：
摘要：
Intervertebral disc (IVD) degeneration and associated low back pain (LBP) remains a major burden to our society without significant improvements in treatment strategies or patient's quality of life. While the recent cell-transplantation studies for treatment of degenerative disc disease have shown promising results, to better gauge the success and functional outcomes of these therapies, it is crucial to understand if transplanted cells give rise to healthy nucleus pulposus (NP) tissue. NP cell phenotype is unique and is defined by expression of a characteristic set of markers that reflect specialized physiology and function. This review summarizes phenotypic markers that mirror the unique physiology and function of NP cells and their progenitors and should be considered to when measuring outcomes of cell-based therapies to treat disc degeneration.
引用
收藏
页码：307 / 316
页数：10
相关论文
共 122 条
[1]   Porcine Intervertebral Disc Repair Using Allogeneic Juvenile Articular Chondrocytes or Mesenchymal Stem Cells [J].
Acosta, Frank L., Jr. ;
Metz, Lionel ;
Adkisson, Huston Davis ;
Liu, Jane ;
Carruthers-Liebenberg, Ellen ;
Milliman, Curt ;
Maloney, Michael ;
Lotz, Jeffrey C. .
TISSUE ENGINEERING PART A, 2011, 17 (23-24) :3045-3055
[2]   Normoxic stabilization of HIF-1α drives glycolytic metabolism and regulates aggrecan gene expression in nucleus pulposus cells of the rat intervertebral disk [J].
Agrawal, Amit ;
Guttapalli, Asha ;
Narayan, Srinivas ;
Albert, Todd J. ;
Shapiro, Irving M. ;
Risbud, Makarand V. .
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 2007, 293 (02) :C621-C631
[3]   Cited2 Modulates Hypoxia-Inducible Factor-Dependent Expression of Vascular Endothelial Growth Factor in Nucleus Pulposus Cells of the Rat Intervertebral Disc [J].
Agrawal, Amit ;
Gajghate, Sachin ;
Smith, Harvey ;
Anderson, D. Greg ;
Albert, Todd J. ;
Shapiro, Irving M. ;
Risbud, Makarand V. .
ARTHRITIS AND RHEUMATISM, 2008, 58 (12) :3798-3808
[4]  
[Anonymous], 2014, INTERVERTEBRAL DISC
[5]   The human lumbar intervertebral disc - Evidence for changes in the biosynthesis and denaturation of the extracellular matrix with growth, maturation, ageing, and degeneration [J].
Antoniou, J ;
Steffen, T ;
Nelson, F ;
Winterbottom, N ;
Hollander, AP ;
Poole, RA ;
Aebi, M ;
Alini, M .
JOURNAL OF CLINICAL INVESTIGATION, 1996, 98 (04) :996-1003
[6]   Oxygen and lactate concentrations measured in vivo in the intervertebral discs of patients with scoliosis and back pain [J].
Bartels, EM ;
Fairbank, JCT ;
Winlove, CP ;
Urban, JPG .
SPINE, 1998, 23 (01) :1-7
[7]   Isolation and Characterization of Mesenchymal Stromal Cells From Human Degenerated Nucleus Pulposus Comparison With Bone Marrow Mesenchymal Stromal Cells From the Same Subjects [J].
Blanco, Juan F. ;
Graciani, Ignacio F. ;
Sanchez-Guijo, Fermin M. ;
Muntion, Sandra ;
Hernandez-Campo, Pilar ;
Santamaria, Carlos ;
Carrancio, Soraya ;
Barbado, Maria-Victoria ;
Cruz, Graciela ;
Gutierrez-Cosio, Silvia ;
Herrero, Carmen ;
San Miguel, Jesus F. ;
Brinon, Jesus G. ;
del Canizo, Maria-Consuelo .
SPINE, 2010, 35 (26) :2259-2265
[8]   Integrin-mediated interactions with extracellular matrix proteins for nucleus pulposus cells of the human intervertebral disc [J].
Bridgen, D. T. ;
Gilchrist, C. L. ;
Richardson, W. J. ;
Isaacs, R. E. ;
Brown, C. R. ;
Yang, K. L. ;
Chen, J. ;
Setton, L. A. .
JOURNAL OF ORTHOPAEDIC RESEARCH, 2013, 31 (10) :1661-1667
[9]   The Presence of Local Mesenchymal Progenitor Cells in Human Degenerated Intervertebral Discs and Possibilities to Influence These In Vitro: A Descriptive Study in Humans [J].
Brisby, Helena ;
Papadimitriou, Nikolaos ;
Brantsing, Camilla ;
Bergh, Peter ;
Lindahl, Anders ;
Henriksson, Helena Barreto .
STEM CELLS AND DEVELOPMENT, 2013, 22 (05) :804-814
[10]   SPINE UPDATE - AGING AND DEGENERATION OF THE HUMAN INTERVERTEBRAL DISC [J].
BUCKWALTER, JA .
SPINE, 1995, 20 (11) :1307-1314
12345678910