Dextran-based therapeutic nanoparticles for hepatic drug delivery

被引：27

作者：

Foerster, Friedrich ^{[1
,2
,3
]}

Bamberger, Denise ^{[4
]}

Schupp, Jonathan ^{[5
]}

Weilbaecher, Martin ^{[5
]}

Kaps, Leonard ^{[1
,2
]}

Strobl, Stephanie ^{[1
,2
]}

Radi, Lydia ^{[4
]}

Diken, Mustafa ^{[6
]}

Strand, Dennis ^{[3
]}

Tuettenberg, Andrea ^{[5
]}

Wich, Peter R. ^{[4
]}

Schuppan, Detlef ^{[1
,2
,7
]}

机构：

[1] Johannes Gutenberg Univ Mainz, Univ Med Ctr, Inst Translat Immunol, Langenbeckstr 1, D-55131 Mainz, Germany

[2] Johannes Gutenberg Univ Mainz, Univ Med Ctr, Res Ctr Immunotherapy FZI, Langenbeckstr 1, D-55131 Mainz, Germany

[3] Univ Med Ctr, Dept Med 1, Mainz, Germany

[4] Johannes Gutenberg Univ Mainz, Inst Pharm & Biochem, Staudingerweg 5, Mainz, Germany

[5] Univ Med Ctr, Dept Dermatol, Mainz, Germany

[6] Johannes Gutenberg Univ Mainz gGmbH, Univ Med Ctr, TRON Translat Oncol, Freiligrathstr 2, D-55131 Mainz, Germany

[7] Harvard Med Sch, Beth Israel Deaconess Med Ctr, Div Gastroenterol, Boston, MA 02115 USA

来源：

NANOMEDICINE | 2016年 / 11卷 / 20期

关键词：

biodegradable; dendritic cell; dextran; drug delivery; liver targeting; macrophage; nanoparticles; PEGylation; siRNA; PARTICLES; PEG;

D O I：

10.2217/nnm-2016-0156

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Aim: Evaluation of dextran-based nanoparticles (DNP) as a drug delivery system to target myeloid cells of the liver. Materials & methods: DNP were synthesized and optionally PEGylated. Their toxicity and cellular uptake were studied in vitro. Empty and siRNA-carrying DNP were tested in vivo with regard to biodistribution and cellular uptake. Results: In vitro, DNP were taken up by cells of the myeloid lineage without compromising their viability. In vivo, empty and siRNA-carrying DNP distributed to the liver where a single treatment addressed approximately 70% of macrophages and dendritic cells. Serum parameters indicated no in vivo toxicity. Conclusion: DNP are multifunctional liver-specific drug carriers which lack toxic side effects and may be utilized in clinical applications targeting liver macrophages.

引用

页码：2663 / 2677

页数：15

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