Direct measurement of ATP in seawater and application of ATP to monitor bacterial growth potential in SWRO pre-treatment systems

被引:18
作者
Abushaban, Almotasembellah [1 ,2 ]
Mangal, M. Nasir [1 ]
Salinas-Rodriguez, Sergio G. [1 ]
Nnebuo, Chidiebere [1 ]
Mondal, Subhanjan [3 ]
Goueli, Said A. [3 ,4 ]
Schippers, Jan C. [1 ]
Kennedy, Maria D. [1 ,2 ]
机构
[1] UNESCO IHE Inst Water Educ, Environm Engn & Water Technol Dept, Westvest 7, NL-2611 AX Delft, Netherlands
[2] Delft Univ Technol, Stevinweg 1, NL-2628 CN Delft, Netherlands
[3] Promega Corp, 2800 Woods Hollow Rd, Madison, WI 53711 USA
[4] Univ Wisconsin, Sch Med, Madison, WI USA
关键词
Adenosine triphosphate; Seawater; Bacterial growth potential; Reverse osmosis; Desalination; Biofouling; ASSIMILABLE ORGANIC-CARBON; ADENOSINE-TRIPHOSPHATE ATP; GRANULAR ACTIVATED CARBON; DRINKING-WATER; AOC; STABILITY; BIOMASS; CELL; PH;
D O I
10.5004/dwt.2017.21783
中图分类号
TQ [化学工业];
学科分类号
0817 ;
摘要
The use of adenosine triphosphate (ATP) to monitor bacterial growth potential of seawater is currently not possible as ATP cannot be accurately measured at low concentration in seawater using commercially available luciferase-based ATP detection. The limitation is due to interference of salt with the luciferin-luciferase reaction, which inhibits light production. This research demonstrates that new reagents developed for (i) ATP extraction from microbial cells and (ii) ATP detection in seawater are able to reliably detect Microbial ATP as low as 0.3 ng L-1 in seawater. The luminescence signal of the new detection reagent is significantly higher (> 20 times) than the luminescence signal of the freshwater reagent, when applied in seawater. ATP can now be used to monitor bacterial growth potential (BGP) through pre-treatment trains of seawater reverse osmosis (SWRO) plants. The level of detection of the new BGP test is significantly lower than the estimated threshold value required to prevent biofouling in SWRO systems. The new reagents have been used to monitor Microbial ATP in coastal North Sea water. Moreover, Microbial ATP has been applied to monitor the bacterial growth potential (using indigenous bacteria) through the pre-treatment train of an SWRO desalination plant. A significant reduction (> 55%) of the bacterial growth potential was found through the dual media filtration with 4.5 mg-Fe(III) L-1 coagulant. Overall, the new reagents can detect low Microbial ATP concentrations in seawater and can be used to monitor bacterial growth potential in seawater desalination plants.
引用
收藏
页码:91 / 101
页数:11
相关论文
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