Enhancement of fluorescence quenching and exciplex formation in DNA major groove by double incorporation of modified fluorescent deoxyuridines

被引：4

作者：

Tanaka, Makiko ^{[1
,2
]}

Oguma, Kazuhiro ^{[1
,2
]}

Saito, Yoshio ^{[1
]}

Saito, Isao ^{[2
]}

机构：

[1] Nihon Univ, Sch Engn, Dept Chem Biol & Appl Chem, Koriyama, Fukushima 9638642, Japan

[2] Nihon Univ, Sch Engn, NEWCAT Inst, Koriyama, Fukushima 9638642, Japan

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2012年 / 22卷 / 12期

关键词：

DNA; Major groove; Modified nucleoside; Ethynyl linker; Exciplex; SUPRAMOLECULAR SCAFFOLD; HELICAL ARRANGEMENT; DUPLEX FORMATION; ENERGY-TRANSFER; PYRENE; OLIGONUCLEOTIDES; STABILIZATION;

D O I：

10.1016/j.bmcl.2012.04.067

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

5-(1-Naphthalenylethynyl)-2'-deoxyuridine (U-N) and 5-[(4-cyano-1-naphthalenyl) ethynyl]-2'-deoxyuridine (U-CN) were synthesized and incorporated into oligodeoxynucleotides. Fluorescence emissions of modified duplexes containing double U-N were efficiently quenched depending upon the sequence pattern of the naphthalenes in DNA major groove, as compared to the duplex possessing single U-N. When one of the naphthalene moieties has a cyano substituent, the exciplex emission from the chromophores in DNA major groove was observed at longer wavelength. (C) 2012 Elsevier Ltd. All rights reserved.

引用

收藏

页码：4103 / 4105

页数：3

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