Determination of the amino acid sequence of the moult-inhibiting hormone from the edible crab, Cancer pagurus

被引：40
作者：
Chung, JS
Wilkinson, MC
Webster, SG
机构：
[1] UNIV WALES,SCH BIOL SCI,BANGOR,GWYNEDD,WALES
[2] UNIV LIVERPOOL,DEPT BIOCHEM,LIVERPOOL L69 3BX,MERSEYSIDE,ENGLAND
来源：
NEUROPEPTIDES | 1996年 / 30卷 / 01期
基金：
英国生物技术与生命科学研究理事会;
关键词：
D O I：
10.1016/S0143-4179(96)90061-X
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
Putative moult-inhibiting hormone (MIH) from sinus glands of the edible crab Cancer pagurus was characterized by high-performance liquid chromatography, followed by fractional bioassay (inhibition of ecdysteroid synthesis by Y-organs) and immunoassay (using antisera raised against Carcinus MIH). This peptide was fully sequenced by automated Edman degradation of endoproteinase-derived fragments. C. pagurus MIH is a 78 residue peptide (M(r) 9194), with free N- and C-termini and three intrachain disulphide bridges. Comparison with previously published MIH sequences confirms a high degree of sequence identity (c. 80%), supporting the view that brachyurans (crabs), possess distinct, structurally similar MIH neuropeptides.
引用
收藏
页码：95 / 101
页数：7
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