Genetic characterisation of olive trees from Madeira Archipelago using flow cytometry and microsatellite markers

被引：38

作者：

Brito, Gina ^{[1
]}

Loureiro, Joao

Lopes, Tina

Rodriguez, Eleazar

Santos, Conceicao

机构：

[1] Univ Aveiro, Lab Biotechnol & Cyt, CESAM, P-3810193 Aveiro, Portugal

来源：

GENETIC RESOURCES AND CROP EVOLUTION | 2008年 / 55卷 / 05期

关键词：

genetic diversity; genome size; nuclear DNA content; Olea sp; Oleaceae; SSR markers;

D O I：

10.1007/s10722-007-9268-8

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

In this study, native olive plants from Olea maderensis ( O. europaea ssp. cerasiformis) and O. cerasiformis ( O. europaea ssp. guanchica), wild olives (O. europaea ssp. europaea var. sylvestris) and cultivated olives (O. europaea ssp. europaea var. europaea) were analysed with respect to genome size and microsatellite markers. The mean nuclear DNA content of O. maderensis was estimated as 5.97 +/- 0.191 pg/2C, while the remaining studied taxa presented mean genome sizes ranging from 2.99 to 3.18 pg/2C. These data and the obtained simple sequence repeats (SSR) profiles, i.e., with 2-4 alleles in O. maderensis and a maximum of two alleles in the other taxa, enabled the identification of a new ploidy level, tetraploidy, for a species belonging to the Olea genus. Cluster analysis of the microsatellite data revealed a clear separation of each species in different clusters and a high genetic dissimilarity could be observed among genotypes belonging to different species. This work contributed to a better characterization of olive species and the obtained data can be helpful to support taxonomic studies, and to develop germplasm preservation strategies in endangered populations of O. maderensis from Madeira Archipelago.

引用

页码：657 / 664

页数：8

共 27 条

[1]

ANDRADA EC, 1990, REPOVOAMENTO FLOREST

[2]

[Anonymous], 1992, FLORA MADEIRA INTERE

[3] High genetic diversity and clonal growth in relict populations of Olea europaea subsp laperrinei (Oleaceae) from Hoggar, Algeria [J].

Baali-Cherif, D ;

Besnard, G .

ANNALS OF BOTANY, 2005, 96 (05) :823-830

[4] Nuclear genome size and genomic distribution of ribosomal DNA in Musa and Ensete (Musaceae):: taxonomic implications [J].

Bartos, J ;

Alkhimova, O ;

Dolezelová, M ;

De Langhe, E ;

Dolezel, J .

CYTOGENETIC AND GENOME RESEARCH, 2005, 109 (1-3) :50-57

[5] Comparative study of the discriminating capacity of RAPD, AFLP and SSR markers and of their effectiveness in establishing genetic relationships in olive [J].

Belaj, A ;

Satovic, Z ;

Cipriani, G ;

Baldoni, L ;

Testolin, R ;

Rallo, L ;

Trujillo, I .

THEORETICAL AND APPLIED GENETICS, 2003, 107 (04) :736-744

[6] Microsatellite markers isolated in olive (Olea europaea L.) are suitable for individual fingerprinting and reveal polymorphism within ancient cultivars [J].

Cipriani, G ;

Marrazzo, MT ;

Marconi, R ;

Cimato, A ;

Testolin, R .

THEORETICAL AND APPLIED GENETICS, 2002, 104 (2-3) :223-228

[7] Isolation and characterization of polymorphic microsatellites in olive (Olea europaea L.) and their transferability to other genera in the Oleaceae [J].

De La Rosa, R ;

James, CM ;

Tobutt, KR .

MOLECULAR ECOLOGY NOTES, 2002, 2 (03) :265-267

[8] Nuclear DNA content and genome size of trout and human [J].

Dolezel, J ;

Bartos, J ;

Voglmayr, H ;

Greilhuber, J .

CYTOMETRY PART A, 2003, 51A (02) :127-128

[9] Plant genome size estimation by flow cytometry:: Inter-laboratory comparison [J].

Dolezel, J ;

Greilhuber, J ;

Lucretti, S ;

Meister, A ;

Lysák, MA ;

Nardi, L ;

Obermayer, R .

ANNALS OF BOTANY, 1998, 82 :17-26

[10] RAPID FLOW CYTOMETRIC ANALYSIS OF THE CELL-CYCLE IN INTACT PLANT-TISSUES [J].

GALBRAITH, DW ;

HARKINS, KR ;

MADDOX, JM ;

AYRES, NM ;

SHARMA, DP ;

FIROOZABADY, E .

SCIENCE, 1983, 220 (4601) :1049-1051

← 1 2 3 →