Development of a multiplex PCR assay for the identification of Staphylococcus aureus enterotoxigenic strains isolated from milk and dairy products

被引：120
作者：
Cremonesi, P
Luzzana, M
Brasca, M
Morandi, S
Lodi, R
Vimercati, C
Agnellini, D
Caramenti, G
Moroni, P
Castiglioni, B
机构：
[1] Univ Milan, Dept Biomed Sci & Technol, I-20090 Milan, Italy
[2] Italian Natl Res Council, Inst Sci Food Prod, Milan, Italy
[3] Univ Milan, Dept Anim Pathol, Milan, Italy
[4] Italian Natl Res Council, Inst Biomed Technol, Milan, Italy
[5] Italian Natl Res Council, Inst Agr Biol & Biotechnol, Milan, Italy
来源：
MOLECULAR AND CELLULAR PROBES | 2005年 / 19卷 / 05期
关键词：
enterotoxigenic Staphylococcus aureus; multiplex PCR; milk; dairy products;
D O I：
10.1016/j.mcp.2005.03.002
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A multiplex PCR for the simultaneous detection of Staphylococcus aureus 23S rRNA, the coagulase and thermonuclease genes as well as the enterotoxin genes sea, sec, sed, seg, seh, sei, sej, sel was developed. The method was used to determine the presence of enterotoxigenic types for 93 S. aureus strains isolated from milk and dairy products. The data obtained by mPCR resulted comparable to those obtained by immunoassay methods. In addition, the mPCR assays also amplified some se genes, whose toxins are undetectable by immunoassay. Multiplex amplification can be obtained starting from 1 pg of DNA, showing the excellent specificity and high sensitivity of the assay. (C) 2005 Elsevier Ltd. All rights reserved.
引用
收藏
页码：299 / 305
页数：7
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