Holo-Ni2+ Helicobacter pylori NikR contains four square-planar nickel-binding sites at physiological pH

被引：21

作者：

Benini, Stefano ^{[2
]}

Cianci, Michele ^{[3
]}

Ciurli, Stefano ^{[1
]}

机构：

[1] Univ Bologna, Bioorgan Chem Lab, I-40127 Bologna, Italy

[2] Free Univ Bolzano, Fac Sci & Technol, I-39100 Bolzano, Italy

[3] DESY, European Mol Biol Lab, D-22603 Hamburg, Germany

来源：

DALTON TRANSACTIONS | 2011年 / 40卷 / 31期

关键词：

TRANSCRIPTION FACTOR NIKR; ESCHERICHIA-COLI; STRUCTURAL BASIS; METAL-BINDING; DNA; ACTIVATION; REPRESSOR; IONS; NI2+;

D O I：

10.1039/c1dt11107h

中图分类号：

O61 [无机化学];

学科分类号：

070301 ; 081704 ;

摘要：

The crystal structure of Helicobacter pylori holo-NikR, a Ni2+-dependent transcription factor, determined at pH 7.3, shows four square-planar nickel-binding sites, involving one cysteinate and three histidine ligands. This observation reconciles previous inconsistencies among calorimetric data, structural information at non-physiological pH, and computational studies.

引用

页码：7831 / 7833

页数：3

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