The mechanism of neutral red-mediated microbial electrosynthesis in Escherichia coli: menaquinone reduction

被引：65

作者：

Harrington, Timothy D. ^{[1
]}

Tran, Vi N. ^{[1
]}

Mohamed, Abdelrhman ^{[1
]}

Renslow, Ryan ^{[2
]}

Biria, Saeid ^{[1
]}

Orfe, Lisa ^{[3
]}

Call, Douglas R. ^{[3
]}

Beyenal, Haluk ^{[1
]}

机构：

[1] Washington State Univ, Gene & Linda Voiland Sch Chem Engn & Bioengn, Pullman, WA 99164 USA

[2] Pacific NW Natl Lab, Environm Mol Sci Lab, Richland, WA 99352 USA

[3] Washington State Univ, Paul G Allen Sch Global Anim Hlth, Pullman, WA 99164 USA

来源：

BIORESOURCE TECHNOLOGY | 2015年 / 192卷

基金：

美国国家科学基金会;

关键词：

Neutral red; Electron transfer; Bioelectrochemical system; Microbial electrosynthesis; Menaquinone; GENE-EXPRESSION; MEMBRANE; FERMENTATION; HYDROGENASE; SHIFT; WATER;

D O I：

10.1016/j.biortech.2015.06.037

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

The aim of this work was to elucidate the mechanism of mediated microbial electrosynthesis via neutral red from an electrode to fermenting Escherichia coli cultures in a bioelectrochemical system. Chemical reduction of NAD+ by reduced neutral red did not occur as predicted. Instead, neutral red was shown to reduce the menaquinone pool in the inner bacterial membrane. The reduced menaquinone pool altered fermentative metabolite production via the arcB redox-sensing cascade in the absence of terminal electron acceptors. When the acceptors DMSO, fumarate, or nitrate were provided, as many as 19% of the electrons trapped in the reduced acceptors were derived from the electrode. These results demonstrate the mechanism of neutral red-mediated microbial electrosynthesis during fermentation as well as how neutral red enables microbial electrosynthesis of reduced terminal electron acceptors. (C) 2015 Elsevier Ltd. All rights reserved.

引用

页码：689 / 695

页数：7

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