The use of BDNF to enhance the patency rate of small-diameter tissue-engineered blood vessels through stem cell homing mechanisms

被引：55

作者：

Zeng, Wen ^{[1
]}

Wen, Can ^{[1
]}

Wu, Yangxiao ^{[1
]}

Li, Li ^{[1
]}

Zhou, Zhenhua ^{[2
]}

Mi, Jianhong ^{[1
]}

Chen, Wen ^{[1
]}

Yang, Mingcan ^{[1
]}

Hou, Chunli ^{[1
]}

Sun, Jiansen ^{[1
]}

Zhu, Chuhong ^{[1
]}

机构：

[1] Third Mil Med Univ, Key Lab Biomech & Tissue Engn Chongqing, Dept Anat, Chongqing 400038, Peoples R China

[2] Third Mil Med Univ, Southwest Hosp, Dept Neurol, Chongqing 400038, Peoples R China

来源：

BIOMATERIALS | 2012年 / 33卷 / 02期

基金：

美国国家科学基金会;

关键词：

Tissue-engineered blood vessels; Endothelial progenitor cells; Endothelialization; Vascular matrix material; Brain-derived neurotrophic factors; Patency rate; ENDOTHELIAL PROGENITOR CELLS; NEUROTROPHIC FACTOR; VASCULAR GRAFTS; REVASCULARIZATION; ACTIVATION; POTENTIATION; MOBILIZATION; EXPRESSION; CONDUITS; SURVIVAL;

D O I：

10.1016/j.biomaterials.2011.09.066

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

The patency rate of small-diameter tissue-engineered blood vessels is the determinant for their application in coronary artery bypass grafting. The coronary artery is innervated by vagus nerves. The origin of vagus nerves is rich in brain-derived neurotrophic factors (BDNF). We have investigated whether BDNF could improve the patency rate of small-diameter tissue-engineered blood vessels through promoting stem cell homing and paracrine activity. In vitro, we isolated early and late endothelial progenitor cells (EPCs) and found BDNF could promote single clone formation and paracrine function of EPCs, and could also induce the proliferation, migration and differentiation of late EPCs. BDNF could enhance the capturing of EPCs in parallel-plate flow chamber. Flow cytometric analysis and laser-scanning confocal microscope showed BDNF could enhance the mobilization and homing of C57BL/6 mouse EPCs after wire injury. Based on it, BDNF was coupled to the lumen surface of the blood vessel matrix material incubated with collagen through SPDP to construct BDNF-modified small-diameter tissue-engineered blood vessel. The blood vessel patency rate was significantly higher than that of control group 8 weeks after implantation in rats and the endothelialization level was superior to control. These results demonstrate that BDNF can effectively improve patency of small-diameter tissue-engineered blood vessels through stem cell homing and paracrine, and it is expected to play an important role in the construction of substitutes for coronary artery bypass grafting. (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：473 / 484

页数：12

共 38 条

[1] Binding of a model regulator of complement activation (RCA) to a biomaterial surface: surface-bound factor H inhibits complement activation [J].

Andersson, J ;

Larsson, R ;

Richter, R ;

Ekdahl, KN ;

Nilsson, B .

BIOMATERIALS, 2001, 22 (17) :2435-2443

[2] Lipoprotein-associated phospholipase A2, high-sensitivity C-reactive protein, and risk for incident coronary heart disease in middle-aged men and women in the Atherosclerosis Risk in Communities (ARIC) study [J].

Ballantyne, CM ;

Hoogeveen, RC ;

Bang, H ;

Coresh, J ;

Folsom, AR ;

Heiss, G ;

Sharrett, AR .

CIRCULATION, 2004, 109 (07) :837-842

[3] Gene expression profiling of facilitated L-LTP in VP16-CREB mice reveals that BDNF is critical for the maintenance of LTP and its synaptic capture [J].

Barco, A ;

Patterson, S ;

Alarcon, JM ;

Gromova, P ;

Mata-Roig, M ;

Morozov, A ;

Kandell, ER .

NEURON, 2005, 48 (01) :123-137

[4]

Bauters Christophe, 2008, Rev Prat, V58, P1523

[5] Gating of BDNF-induced synaptic potentiation by cAMP [J].

Boulanger, L ;

Poo, MM .

SCIENCE, 1999, 284 (5422) :1982-1984

[6] Cardiovascular Actions of Neurotrophins [J].

Caporali, Andrea ;

Emanueli, Costanza .

PHYSIOLOGICAL REVIEWS, 2009, 89 (01) :279-308

[7] Transcriptional Upregulation of Brain-Derived Neurotrophic Factor in Rostral Ventrolateral Medulla by Angiotensin II Significance in Superoxide Homeostasis and Neural Regulation of Arterial Pressure [J].

Chan, Samuel H. H. ;

Wu, Chih-Wei J. ;

Chang, Alice Y. W. ;

Hsu, Kuei-Sen ;

Chan, Julie Y. H. .

CIRCULATION RESEARCH, 2010, 107 (09) :1127-U180

[8] Readily Available Tissue-Engineered Vascular Grafts [J].

Dahl, Shannon L. M. ;

Kypson, Alan P. ;

Lawson, Jeffrey H. ;

Blum, Juliana L. ;

Strader, Justin T. ;

Li, Yuling ;

Manson, Roberto J. ;

Tente, William E. ;

DiBernardo, Louis ;

Hensley, M. Taylor ;

Carter, Riley ;

Williams, Tiare P. ;

Prichard, Heather L. ;

Dey, Margaret S. ;

Begelman, Keith G. ;

Niklason, Laura E. .

SCIENCE TRANSLATIONAL MEDICINE, 2011, 3 (68)

[9] Tissue Engineering Small-Diameter Vascular Grafts: Preparation of a Biocompatible Porcine Ureteric Scaffold [J].

Derham, Chris ;

Yow, Heng ;

Ingram, Joanne ;

Fisher, John ;

Ingham, Eileen ;

Korrosis, Sotirios A. ;

Homer-Vanniasinkam, Shervanthi .

TISSUE ENGINEERING PART A, 2008, 14 (11) :1871-1882

[10]

Donovan MJ, 2000, DEVELOPMENT, V127, P4531

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