The rapid determination of gamma-aminobutyric acid

被引:0
作者
Zhang, GJ [1 ]
Bown, AW [1 ]
机构
[1] BROCK UNIV, DEPT BIOL SCI, ST CATHARINES, ON L2S 3A1, CANADA
关键词
Glycine max; Leguminosae; soybean; gamma-aminobutyric acid (GABA) determination; stress;
D O I
暂无
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A rapid procedure for the extraction and assay of gamma-aminobutyric acid (GABA) is described. The extraction procedure prevents rapid GABA accumulation during the sampling of tissue for analysis. It also removes over 95% of pigments absorbing at 340 nm which otherwise reduce the sensitivity of the spectrophotometric coupled enzyme assay. The absorbance increase at 340 nm was linear for GABA levels ranging from 10 to 100 nmol per cuvette. Copyright (C) 1997 Elsevier Science Ltd.
引用
收藏
页码:1007 / 1009
页数:3
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