Detection of telomerase activity utilizing energy transfer primers: Comparison with gel- and ELISA-Based detection

被引：44

作者：

Uehara, H ^{[1
]}

Nardone, G ^{[1
]}

Nazarenko, I ^{[1
]}

Hohman, RJ ^{[1
]}

机构：

[1] Intergen Co, Gaithersburg, MD 20877 USA

来源：

BIOTECHNIQUES | 1999年 / 26卷 / 03期

关键词：

D O I：

10.2144/99263pf01

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We have developed a closed-tube format telomeric repent amplification protocol (TRAP) assay for direct quantification of telomerase activity within the PCR vessel. The assay utilizes energy transfer (ET) primers, which emit fluorescence only upon incorporation into PCR products. This novel ET primer system (Amplifluor(TM) primers) has major advantages over existing detection methods because it eliminates the need for post-PCR processing and the time reduces greatly the risk of carryover contamination and the time required for the sample analysis. The assay is as sensitive, specific and quantitative as the polyacrylamide gel-based or ELISA-based TRAP assay.

引用

页码：552 / +

页数：6

共 18 条

[1]

BACCHETTI S, 1995, INT J ONCOL, V7, P423

[2] STRUCTURE AND FUNCTION OF TELOMERES [J].