Characterization and optimization of mesoporous magnetic nanoparticles for immobilization and enhanced performance of porcine pancreatic lipase

被引:13
作者
Shao, Yong-Bo [1 ]
Jing, Tao [1 ]
Tian, Jing-Zhi [1 ]
Zheng, Yong-Jie [1 ]
Shang, Ming-Hui [1 ]
机构
[1] Qiqihar Univ, Coll Chem & Chem Engn, Qiqihar 161006, Peoples R China
来源
CHEMICAL PAPERS | 2015年 / 69卷 / 10期
基金
中国国家自然科学基金;
关键词
Fe3O4; nanoparticles; porcine pancreas lipase; covalent attachment; physical adsorption; cross-linking; IRON-OXIDE NANOPARTICLES; CANDIDA-RUGOSA LIPASE; CATALYTIC PERFORMANCE; SILICA; ADSORPTION; PROTEIN; ACID; COMPOSITE; CHITOSAN; STABILIZATION;
D O I
10.1515/chempap-2015-0142
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
In this paper, Fe3O4 nanoparticles coated with mesoporous silica were prepared successfully, noted as Fe3O4 at the mobile composition of matter No. 41 (MCM-41). Also, Fe3O4 at MCM-41 was grafted by both 3-aminopropyltriethoxysilane (APTS) and 3-chloropropyltriethoxysilane (CPS), noted as Fe3O4 at MCM-41/APTS and Fe3O4 at MCM-41/CPS. The compounds were characterized by X-ray powder diffraction, scanning electron microscopy, transmission electron microscopy, Fourier transform infrared spectroscopy, vibrating sample magnetometry, thermogravimetry and N-2 adsorption/desorption. Then, the enzyme, porcine pancreas lipase (PPL), was immobilized onto these modified nanoparticles by covalent attachment, physical adsorption and cross-linking, noted as Fe3O4 at MCM-41/CPS-PPL, Fe3O4 at MCM-41-PPL and Fe3O4 at MCM-41/APTS-PPL, respectively. The results showed that Fe3O4 at MCM-41/CPS was the best nanomaterial for PPL immobilization, exhibiting enhanced immobilization efficiency (maximum 96 %), maximum relative activity (up to 96 %), high stability and reusability (83 % 56 days and 86.7 % ten cycles). Additionally, it offered some other advantages, such as easy recycling and reuse, complying with the trend of green chemistry. Therefore, Fe3O4 at MCM-41/CPS in combination with a relevant method can be proposed for commercial applications. (C) 2015 Institute of Chemistry, Slovak Academy of Sciences
引用
收藏
页码:1298 / 1311
页数:14
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