Crassostrea gigas exposure to the dinoflagellate Prorocentrum lima: Histological and gene expression effects on the digestive gland

被引：27

作者：

de Jesus Romero-Geraldo, Reyna ^{[1
]}

Garcia-Lagunas, Norma ^{[2
]}

Yolanda Hernandez-Saavedra, Norma ^{[2
]}

机构：

[1] Inst Tecnol La Paz, Blvd Forjadores Baja Calif Sur 4720, La Paz 23080, Baja California, Mexico

[2] Ctr Invest Biol Noroeste CIBNOR, CIBNOR Mol Genet Lab, Ave Inst Politecn Nacl 195,Apartado Postal128, La Paz 23096, Baja California, Mexico

来源：

MARINE ENVIRONMENTAL RESEARCH | 2016年 / 120卷

关键词：

Crassostrea gigas; Cell cycle; Cytoskeleton; Inflammation; Prorocentrum lima; Digestive gland; OKADAIC ACID; MYTILUS-GALLOPROVINCIALIS; RUDITAPES-PHILIPPINARUM; ALEXANDRIUM-CATENELLA; PERKINSUS-OLSENI; SUMMER MORTALITY; VARYING PARASITE; HARMFUL-ALGA; GUT PASSAGE; IN-VITRO;

D O I：

10.1016/j.marenvres.2016.07.011

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Bivalve mollusks bioaccumulate toxins via ingestion of toxic dinoflagellates. In this study, Crassostrea gigas was used to investigate the effects related to Prorocentrum lima exposure. Oysters were fed with three diets Isochrysi. galbana (2 x 10(6) cell mL(-1)) control treatment; algal mix of I. galbana (2 x 10(6)) and P. lima (3 x 103 cell mL(-1)); and P. lima alone (3 x 103 cell mL(-1)). Feeding behavior changes, histopathological alterations, and expression patterns changes of genes involved in cell cycle (p21, cafp55, p53), cytoskeleton (tub, act), and inflammatory process (casp1) were evaluated. Results,indicated that the presence of diarrheic shellfish poisoning by P. lima cells decreased the clearance rate (p < 0.05), induced structural loss, significantly decreased tubule area of the digestive gland (p < 0.05), and up -regulated in expression all gene (p < 0.05), suggesting that toxic cells might trigger inflammatory tissue process, disturb cell cycle and cytoskeleton representing a risk to oysters integrity. (C) 2016 Elsevier Ltd. All rights reserved.

引用

页码：93 / 102

页数：10

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