Femtosecond laser nanoaxotomy lab-on-achip for in vivo nerve regeneration studies

被引：152

作者：

Guo, Samuel X. ^{[1
]}

Bourgeois, Frederic ^{[1
]}

Chokshi, Trushal ^{[2
]}

Durr, Nicholas J. ^{[1
]}

Hilliard, Massimo A. ^{[3
]}

Chronis, Nikos ^{[2
]}

Ben-Yakar, Adela ^{[1
]}

机构：

[1] Univ Texas Austin, Dept Mech Engn, Austin, TX 78712 USA

[2] Univ Michigan, Dept Mech Engn, Ann Arbor, MI 48109 USA

[3] Univ Queensland, Queensland Brain Inst, Brisbane, Qld 4072, Australia

来源：

NATURE METHODS | 2008年 / 5卷 / 06期

关键词：

D O I：

10.1038/NMETH.1203

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A thorough understanding of nerve regeneration in Caenorhabditis elegans requires performing femtosecond laser nanoaxotomy while minimally affecting the worm. We present a microfluidic device that fulfills such criteria and can easily be automated to enable high-throughput genetic and pharmacological screenings. Using the 'nanoaxotomy' chip, we discovered that axonal regeneration occurs much faster than previously described, and notably, the distal fragment of the severed axon regrows in the absence of anesthetics.

引用

页码：531 / 533

页数：3

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