Selective cancer-killing ability of new efficient porphyrin-based nanophotosensitizer in Lab-on-a-chip system

被引：9

作者：

Tokarska, Katarzyna ^{[1
]}

Bazylinska, Urszula ^{[2
]}

Jastrzebska, Elzbieta ^{[1
]}

Chudy, Michal ^{[1
]}

Dybko, Artur ^{[1
]}

Wilk, Kazimiera A. ^{[2
]}

Brzozka, Zbigniew ^{[1
]}

机构：

[1] Warsaw Univ Technol, Fac Chem, Chair Med Biotechnol, Warsaw, Poland

[2] Wroclaw Univ Sci & Technol, Fac Chem, Dept Organ & Pharmaceut Technol, Wroclaw, Poland

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2019年 / 282卷

关键词：

Photodynamic therapy; Nanophotosensitizer; Lab-on-a-chip; Tetraphenylporphyrin; Oil core nanocapsules; PHOTODYNAMIC THERAPY; GOLD NANOPARTICLES; TETRAPHENYLPORPHYRIN; PHOTOSENSITIZERS;

D O I：

10.1016/j.snb.2018.11.115

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The oil core nanocapsules platform holds great potential to enhance the efficiency and accuracy of anticancer therapies. In this work, we describe the preparation of well-defined multilayer oil core nanocapsules built of oppositely charged polyelectrolytes via a layer-by-layer (LbL) assembly strategy and their use as advanced drug delivery systems for photodynamic therapy (PDT). The prepared nanocapsules show great stability in a physiological environment with uniform morphology and diameters of around 120 nm as disclosed by stability investigation, TEM and DLS analysis. By in vitro cellular experiments based on MTT assays, CLSM, FAGS analysis and ROS detection, we confirmed that tetraphenylporphyrin TPP-loaded multilayer nanocapsules were selectively PDT-active against A549 cancer cells (human lung adenocarcinoma). Cellular uptake results showed that TPP NCs were readily taken up by A549 cells and enhanced TPP uptake was detectable in the NCs-treated cells in comparison to carrier free drug. TPP NCs also elicited intracellular ROS generation, which leads to enhanced toxicity in A549 cells. Toxicological impact of developed nanocapsules was further investigated using newly designed microfluidic Lab-on-a-chip system enabling photodynamic activity studies on three types of cell culture. Our results demonstrate a major potential of TPP-loaded multilayer oil core nanocapsules for PDT therapeutic treatment of a lung cancer.

引用

页码：665 / 674

页数：10

共 34 条

[1] Effect of the preparation method of silver and gold nanoparticles on the photosensitizing properties of tetraphenylporphyrin-amphiphilic polymer-nanoparticle systems [J].