Hemoglobin-Based Oxygen Carrier Microparticles: Synthesis, Properties, and In Vitro and In Vivo Investigations

被引：76

作者：

Xiong, Yu ^{[1
]}

Steffen, Axel ^{[1
]}

Andreas, Kristin ^{[4
,5
]}

Mueller, Susanne ^{[2
,3
]}

Sternberg, Nadine ^{[1
]}

Georgieva, Radostina ^{[1
]}

Baeumler, Hans ^{[1
]}

机构：

[1] Charite, Inst Transfus Med, D-10117 Berlin, Germany

[2] Charite, Dept Expt Neurol, D-10117 Berlin, Germany

[3] Charite, Ctr Stroke Res Berlin, D-10117 Berlin, Germany

[4] Charite, Tissue Engn Lab, D-10117 Berlin, Germany

[5] Charite, Dept Rheumatol & Clin Immunol, Berlin Brandenburg Ctr Regenerat Therapies, D-10117 Berlin, Germany

来源：

BIOMACROMOLECULES | 2012年 / 13卷 / 10期

关键词：

BOVINE HEMOGLOBIN; BLOOD SUBSTITUTES; POLYMERIZED HEMOGLOBIN; PROTEIN ENCAPSULATION; MICROCAPSULES; TRIAL; METHEMOGLOBIN; MULTICENTER; PARTICLES; CAPSULES;

D O I：

10.1021/bm301085x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Bovine hemoglobin microparticles (Hb-MPs) as suitable oxygen carriers are fabricated easily by three key steps: coprecipitation of Hb and CaCO3 to make Hb-CaCO3-microparticles (Hb-CaCO3-MPs), cross-linking by glutaraldehyde (GA) to polymerize the Hb and dissolution of CaCO3 template to obtain pure Hb-MPs. The Hb entrapment efficiency ranged from 8 to 50% corresponding to a hemoglobin quantity per Hb-MP of at least one-third of that in one erythrocyte. The Hb-MPs are spherical, with an average diameter of 3.2 mu m and high oxygen affinity. The methemoglobin level was increased after preparation, but can be reduced to less than 7% with ascorbic acid. Phagocytosis assays showed low immunogenicity of Hb-MPs if the particles were cross-linked with low concentration of GA and treated with sodium borohydride. Magnetite-loaded Hb-MPs circulated up to 4 days after intravenous application.

引用

页码：3292 / 3300

页数：9