On-bead fluorescence assay for serine/threonine kinases

被引：26
作者：
Akita, S [1 ]
Umezawa, N [1 ]
Higuchi, T [1 ]
机构：
[1] Nagoya City Univ, Grad Sch Pharmaceut Sci, Mizuho Ku, Nagoya, Aichi, Japan
来源：
ORGANIC LETTERS | 2005年 / 7卷 / 25期
关键词：
D O I：
10.1021/ol052125k
中图分类号：
O62 [有机化学];
学科分类号：
070303 ; 081704 ;
摘要：
[GRAPHIC] A novel fluorescence-based assay for serine/threonine kinases is described. Base-mediated beta-elimination of the phosphate moiety and the Michael addition of a thiol-containing fluorescent molecule allows convenient and efficient detection of the enzyme activity. This approach may be broadly applicable to various serine/threonine kinases.
引用
收藏
页码：5565 / 5568
页数：4
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