Effect of lignin content on a GH11 endoxylanase acting on glucuronoarabinoxylan-lignin nanocomposites

被引：4
作者：
Boukari, Imen [1 ,2 ]
Remond, Caroline [1 ,2 ]
O'Donohue, Michael [3 ,4 ,5 ]
Chabbert, Brigitte [1 ,2 ]
机构：
[1] INRA, Fractionnement AgroRessources & Environm UMR614, F-51100 Reims, France
[2] Univ Reims, Fractionnement AgroRessources & Environm UMR614, F-51100 Reims, France
[3] Univ Toulouse, INSA, UPS, INP,LISBP, F-31077 Toulouse, France
[4] INRA, Ingn Syst Biol & Procedes UMR792, F-31400 Toulouse, France
[5] CNRS, UMR5504, F-31400 Toulouse, France
来源：
CARBOHYDRATE POLYMERS | 2012年 / 89卷 / 02期
关键词：
Lignin model compounds (DHP); GH11; endoxylanase; Lignin-carbohydrate nanocomposites; Particle size; Enzyme inhibition; Adsorption surface; ENZYMATIC-HYDROLYSIS; POLYMER MODELS; CELLULOSE; BIOMASS; INHIBITION; SOLUBILITY; DHPS; ADSORPTION; MICROSCOPY; CONIFERYL;
D O I：
10.1016/j.carbpol.2012.03.024
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
The effects of lignin content on the activity and action pattern of GH11 endoxylanase from Thermobacillus xylanilyticus were investigated using in vitro reconstituted non-covalent glucuronoarabinoxylan-model lignin (GAX-DHP) nanocomposites. Four types of nanocomposites were prepared, each displaying different lignin contents. Variations in the DHP (model lignin) polymerization process were induced by increasing the coniferyl alcohol concentration. Examination of the morphology of the nanocomposites revealed globular particles enrobed in a matrix. The size of these particles increased in line with the lignin concentration. Physicochemical characterization of the in vitro reconstituted GAX-DHPs strongly suggested that increased particle size is directly related to the solubility and reactivity of coniferyl alcohol, as reflected by changes in the amount of beta-O-4 linkages. Evaluation of the impact of the GH11 endoxylanase on the GAX-DHP nanocomposites revealed a negative correlation between the proportion and organization patterns of DHP in the nanocomposites and enzyme activity. (C) 2012 Elsevier Ltd. All rights reserved.
引用
收藏
页码：423 / 431
页数：9
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