Enzyme immunoassay and liquid chromatography-fluorescence detection for amikacin in raw milk

被引：9

作者：

Chen, Yiqiang ^{[1
]}

Chen, Qian ^{[2
]}

He, Lidong ^{[3
]}

Shang, Bingru ^{[1
]}

Zhang, Liying ^{[1
]}

机构：

[1] China Agr Univ, Coll Anim Sci & Technol, State Key Lab Anim Nutr, Beijing 100193, Peoples R China

[2] China Agr Univ, Coll Vet Med, Dept Basic Vet, Beijing 100193, Peoples R China

[3] China Agr Univ, Coll Sci, Dept Chem, Beijing 100193, Peoples R China

来源：

FOOD CHEMISTRY | 2012年 / 135卷 / 02期

基金：

美国国家科学基金会;

关键词：

Amikacin; Polyclonal antibody; Enzyme immunoassay; Liquid chromatography; Milk; SWINE TISSUES; DERIVATIZATION; GENTAMICIN; KANAMYCIN; ELECTROPHORESIS; TOBRAMYCIN; RESIDUE;

D O I：

10.1016/j.foodchem.2012.05.001

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

An enzyme immunoassay and a liquid chromatography (LC) method for amikacin (AMK) in raw milk were developed in this study. Anti-AMK antibody was prepared by immunizing rabbits with AMK-BSA conjugate. The developed immunoassay exhibited an IC50 value of 1.30 ng/mL and the spiked recoveries at 25-1000 ng/mL ranged from 69.8% to 93.3% with coefficients of variation (CVs) of 8.5-17.6%. For LC analysis, AMK was derivatized with 9-fluorenylmethyl chloroformate, which was followed by C8 column separation and fluorescence detection. By trichloroacetic acid extraction and MCX column purification, the recoveries at spiked concentrations of 500-5000 ng/mL were 80.7-91.3% with CVs less than 6.3%. The two methods can be selectively used for rapidly screening or quantitatively determining AMK in raw milk. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：380 / 385

页数：6

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