Role of microtubules and tea1p in establishment and maintenance of fission yeast cell polarity

被引:69
作者
Sawin, KE [1 ]
Snaith, HA [1 ]
机构
[1] Univ Edinburgh, Inst Cell & Mol Biol, Wellcome Trust Ctr Cell Biol, Edinburgh EH9 3JR, Midlothian, Scotland
关键词
fission yeast; Schizosaccharomyces pombe; microtubules; tea1p; cell polarity;
D O I
10.1242/jcs.00925
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Microtubules and the protein tealp have important roles in regulating cell polarity in the fission yeast Schizosaccharomyces pombe. Here, using combinations of drugs, environmental perturbations and genetic mutants, we demonstrate that once a cell polarity axis is established, microtubules have at best a minor role in maintaining the cortical actin cytoskeleton and the rate and direction of cell growth. In addition, we find that after perturbations that disrupt cell polarity and the cortical actin cytoskeleton, microtubules are not required for re-establishment of polarity per se. However, after such perturbations, the distribution of cytoplasmic microtubules plays an important role in dictating the position of sites of polarity re-establishment. Furthermore, this influence of microtubule distribution on site selection during polarity re-establishment requires the presence of tealp, suggesting that tealp is crucial for coupling microtubule distribution to the regulation of cell polarity. Our results suggest a model in which, at the cellular level, two distinct and separable mechanisms contribute to how tealp regulates site selection during polarity re-establishment. First, tealp remaining at cell tips after cortical depolarization can serve as a cortical landmark for microtubule-independent site selection; second, tealp newly targeted to the cell cortex by association with microtubules can promote the formation of polarity axes de novo.
引用
收藏
页码:689 / 700
页数:12
相关论文
共 40 条
[1]   Control of cell polarity and mitotic spindle positioning in animal cells [J].
Ahringer, J .
CURRENT OPINION IN CELL BIOLOGY, 2003, 15 (01) :73-81
[2]   Tea3p is a cell end marker activating polarized growth in Schizosaccharomyces pombe [J].
Arellano, M ;
Niccoli, T ;
Nurse, P .
CURRENT BIOLOGY, 2002, 12 (09) :751-756
[3]   Pom1p, a fission yeast protein kinase that provides positional information for both polarized growth and cytokinesis [J].
Bähler, J ;
Pringle, JR .
GENES & DEVELOPMENT, 1998, 12 (09) :1356-1370
[4]   Roles of fission yeast tea1p in the localization of polarity factors and in organizing the microtubular cytoskeleton [J].
Behrens, R ;
Nurse, P .
JOURNAL OF CELL BIOLOGY, 2002, 157 (05) :783-793
[5]   Tea2p is a kinesin-like protein required to generate polarized growth in fission yeast [J].
Browning, H ;
Hayles, J ;
Mata, J ;
Aveline, L ;
Nurse, P ;
McIntosh, JR .
JOURNAL OF CELL BIOLOGY, 2000, 151 (01) :15-27
[6]   Targeted movement of cell end factors in fission yeast [J].
Browning, H ;
Hackney, DD ;
Nurse, P .
NATURE CELL BIOLOGY, 2003, 5 (09) :812-818
[7]   CLIP170-like tip1p spatially organizes microtubular dynamics in fission yeast [J].
Brunner, D ;
Nurse, P .
CELL, 2000, 102 (05) :695-704
[8]   COOPERATIVE INTERACTION OF S-POMBE PROTEINS REQUIRED FOR MATING AND MORPHOGENESIS [J].
CHANG, EC ;
BARR, M ;
WANG, Y ;
JUNG, V ;
XU, HP ;
WIGLER, MH .
CELL, 1994, 79 (01) :131-141
[9]   Yeasts make their mark [J].
Chang, F ;
Peter, M .
NATURE CELL BIOLOGY, 2003, 5 (04) :294-299
[10]   Origins of cell polarity [J].
Drubin, DG ;
Nelson, WJ .
CELL, 1996, 84 (03) :335-344