Inhibitory effects of garcinol and pterostilbene on cell proliferation and adipogenesis in 3T3-L1 cells

被引：71

作者：

Hsu, Chin-Lin ^{[1
,3
]}

Lin, Yu-Jyun ^{[2
]}

Ho, Chi-Tang ^{[4
]}

Yen, Gow-Chin ^{[2
]}

机构：

[1] Chung Shan Med Univ, Sch Nutr, Taichung 40201, Taiwan

[2] Natl Chung Hsing Univ, Dept Food Sci & Biotechnol, Taichung 40227, Taiwan

[3] Chung Shan Med Univ Hosp, Dept Nutr, Taichung 40201, Taiwan

[4] Rutgers State Univ, Dept Food Sci, New Brunswick, NJ 08903 USA

来源：

FOOD & FUNCTION | 2012年 / 3卷 / 01期

关键词：

CONJUGATED LINOLEIC-ACID; ADIPOSE CONVERSION; PHENOLIC-ACIDS; PRE-ADIPOCYTES; ADIPONECTIN; APOPTOSIS; OBESITY; SUPPRESSION; FLAVONOIDS; ADIPOKINE;

D O I：

10.1039/c1fo10209e

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The aim of this work was to study the effects of garcinol and pterostilbene on cell proliferation and adipogenesis in 3T3-L1 cells. The results showed that garcinol and pterostilbene decreased the cell population growth and caused cell cycle arrest at the G2/M phase in 3T3-L1 preadipocytes. During adipocyte differentiation, both garcinol and pterostilbene had inhibitory effects on fat droplet formation and triacylglycerol accumulation. The data indicated that garcinol and pterostilbene could inhibit the glycerol-3-phosphate dehydrogenase (GPDH) activity by 97.8 and 61.5%, respectively, as compared to the control. Both garcinol and pterostilbene significantly attenuated the protein expressions of PPAR gamma and C/EBP alpha during 3T3-L1 adipocyte differentiation. Moreover, garcinol and pterostilbene caused an inhibition of lipid accumulation in the 3T3-L1 adipocyte differentiation phase. Garcinol and pterostilbene also significantly up-regulated the gene expression of adiponectin as well as down-regulated the gene expressions of leptin, resistin, and fatty acid synthase (FAS) in 3T3-L1 adipocyte differentiation. In 3T3-L1 adipocytes, garcinol significantly down-regulated the protein expressions of PPAR gamma and FAS as well as up-regulated the protein expressions of adipose triglyceride lipase (ATGL) and adiponectin. Garcinol also significantly up-regulated the gene expression of adiponectin as well as down-regulated the gene expressions of leptin and FAS. These results suggest that garcinol and pterostilbene have anti-adipogenic effects on preadipocytes and adipocytes.

引用

页码：49 / 57

页数：9

共 35 条

[1] Cyclin G2 Regulates Adipogenesis through PPARγ Coactivation [J].

Aguilar, Victor ;

Annicotte, Jean-Sebastien ;

Escote, Xavier ;

Vendrell, Joan ;

Langin, Dominique ;

Fajas, Lluis .

ENDOCRINOLOGY, 2010, 151 (11) :5247-5254

[2] Ajoene Exerts Potent Effects in 3T3-L1 Adipocytes by Inhibiting Adipogenesis and Inducing Apoptosis [J].

Ambati, Suresh ;

Yang, Jeong-Yeh ;

Rayalam, Srujana ;

Park, Hea Jin ;

Della-Fera, Mary Anne ;

Baile, Clifton A. .

PHYTOTHERAPY RESEARCH, 2009, 23 (04) :513-518

[3] ACRP30/adiponectin: an adipokine regulating glucose and lipid metabolism [J].

Berg, AH ;

Combs, TP ;

Scherer, PE .

TRENDS IN ENDOCRINOLOGY AND METABOLISM, 2002, 13 (02) :84-89

[4] The mechanisms of action of PPARs [J].

Berger, J ;

Moller, DE .

ANNUAL REVIEW OF MEDICINE, 2002, 53 :409-435

[5] Pterostilbene Inhibits Colorectal Aberrant Crypt Foci (ACF) and Colon Carcinogenesis via Suppression of Multiple Signal Transduction Pathways in Azoxymethane-Treated Mice [J].

Chiou, Yi-Siou ;

Tsai, Mei-Ling ;

Wang, Ying-Jan ;

Cheng, An-Chin ;

Lai, We-Ming ;

Badmaev, Vladimir ;

Ho, Chi-Tang ;

Pan, Min-Hsiung .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2010, 58 (15) :8833-8841

[6] Plasma concentrations of a novel, adipose-specific protein, adiponectin, in type 2 diabetic patients [J].

Hotta, K ;

Funahashi, T ;

Arita, Y ;

Takahashi, M ;

Matsuda, M ;

Okamoto, Y ;

Iwahashi, H ;

Kuriyama, H ;

Ouchi, N ;

Maeda, K ;

Nishida, M ;

Kihara, S ;

Sakai, N ;

Nakajima, T ;

Hasegawa, K ;

Muraguchi, M ;

Ohmoto, Y ;

Nakamura, T ;

Yamashita, S ;

Hanafusa, T ;

Matsuzawa, Y .

ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, 2000, 20 (06) :1595-1599

[7] Effects of flavonoids and phenolic acids on the inhibition of adipogenesis in 3T3-L1 Adipocytes [J].

Hsu, Chin-Lin ;

Yen, Gow-Chin .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2007, 55 (21) :8404-8410

[8] Gallic acid induces apoptosis in 3T3-L1 pre-adipocytes via a fas- and mitochondrial-mediated pathway [J].

Hsu, Chin-Lin ;

Lo, Wen-Hsieh ;

Yen, Gow-Chin .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2007, 55 (18) :7359-7365

[9] Induction of cell apoptosis in 3T3-L1 pre-adipocytes by flavonoids is associated with their antioxidant activity [J].

Hsu, Chin-Lin ;

Yen, Gow-Chin .

MOLECULAR NUTRITION & FOOD RESEARCH, 2006, 50 (11) :1072-1079

[10] Inhibitory effect of phenolic acids on the proliferation of 3T3-L1 preadipocytes in relation to their antioxidant activity [J].

Hsu, Chin-Lin ;

Huang, Shih-Li ;

Yen, Gow-Chin .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2006, 54 (12) :4191-4197

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