A Third-Generation Hydrogen Peroxide Biosensor Based on Horseradish Peroxidase Immobilized in Carbon Nanotubes/ SBA-15 Film

被引：14

作者：

Fang, Zheng-Hui ^{[1
,2
]}

Lu, Li-Min ^{[1
]}

Zhang, Xiao-Bing ^{[1
]}

Li, Hong-Bo ^{[1
]}

Yang, Bin ^{[1
]}

Shen, Guo-Li ^{[1
]}

Yu, Ru-Qin ^{[1
]}

机构：

[1] Hunan Univ, State Key Lab Chemo Biosensing & Chemometr, Coll Chem & Chem Engn, Changsha 410082, Hunan, Peoples R China

[2] Yueyang Vocat & Tech Coll, Yueyang 414000, Peoples R China

来源：

ELECTROANALYSIS | 2011年 / 23卷 / 10期

基金：

中国国家自然科学基金;

关键词：

Horseradish peroxidase; Carbon nanotubes; Third-generation biosensor; Hydrogen peroxide; Amperometry; DIRECT ELECTROCHEMISTRY; CYTOCHROME-C; AMPEROMETRIC DETERMINATION; ENZYME IMMOBILIZATION; ELECTRON-TRANSFER; METHYLENE GREEN; HEMOGLOBIN; MATRIX; ELECTROCATALYSIS;

D O I：

10.1002/elan.201100211

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A new third-generation biosensor for H2O2 assay was developed on the basis of the immobilization of horseradish peroxidase (HRP) in a nanocomposite film of carbon nanotubes (CNTs)-SBA-15 modified gold electrode. The biological activity of HRP immobilizing in the composite film was characterized by UV-vis spectra. The HRP immobilized in the nanocomposite matrix displayed excellent electrocatalytic activity to the reduction of H2O2. The effects of the experimental variables such as solution pH and working potential were investigated using steady-state amperometry. Under the optimal conditions, the resulting biosensor showed a linear range from 1 mu M to 7 mM and a detection limit of 0.5 mu M (S/N = 3). Moreover, the stability and reproducibility of this biosensor were evaluated with satisfactory results.

引用

页码：2415 / 2420

页数：6

共 46 条

[1] Gold nanoparticles-mesoporous silica composite used as an enzyme immobilization matrix for amperometric glucose biosensor construction [J].

Bai, Yu ;

Yang, Hua ;

Yang, Weiwei ;

Li, Yancai ;

Sun, Changqing .

SENSORS AND ACTUATORS B-CHEMICAL, 2007, 124 (01) :179-186

[2] Direct electrochemistry and electrocatalysis of horseradish peroxidase immobilized in sol-gel-derived ceramic-carbon nanotube nanocomposite film [J].

Chen, Hongjun ;

Dong, Shaojun .

BIOSENSORS & BIOELECTRONICS, 2007, 22 (08) :1811-1815

[3] A glucose biosensor based on enzyme entrapment within polypyrrole films electrodeposited on mesoporous titanium dioxide [J].

Cosnier, S ;

Senillou, A ;

Grätzel, M ;

Comte, P ;

Vlachopoulos, N ;

Renault, NJ ;

Martelet, C .

JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 1999, 469 (02) :176-181

[4] Direct electron transfer and enzymatic activity of hemoglobin in a hexagonal mesoporous silica matrix [J].

Dai, ZH ;

Liu, SQ ;

Ju, HX ;

Chen, HY .

BIOSENSORS & BIOELECTRONICS, 2004, 19 (08) :861-867

[5] Enzyme immobilization in MCM-41 molecular sieve [J].

Diaz, JF ;

Balkus, KJ .

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 1996, 2 (2-3) :115-126

[6]

Fan CH, 2000, ELECTROANAL, V12, P1156, DOI 10.1002/1521-4109(200010)12:14<1156::AID-ELAN1156>3.0.CO

[7]

2-4

[8] Rapid and high-capacity immobilization of enzymes based on mesoporous silicas with controlled morphologies [J].

Fan, J ;

Lei, J ;

Wang, LM ;

Yu, CZ ;

Tu, B ;

Zhao, DY .

CHEMICAL COMMUNICATIONS, 2003, (17) :2140-2141

[9] AMPEROMETRIC SENSORS FOR PEROXIDE, CHOLINE, AND ACETYLCHOLINE BASED ON ELECTRON-TRANSFER BETWEEN HORSERADISH-PEROXIDASE AND A REDOX POLYMER [J].

GARGUILO, MG ;

HUYNH, N ;

PROCTOR, A ;

MICHAEL, AC .

ANALYTICAL CHEMISTRY, 1993, 65 (05) :523-528

[10] Protein electrochemistry using aligned carbon nanotube arrays [J].

Gooding, JJ ;

Wibowo, R ;

Liu, JQ ;

Yang, WR ;

Losic, D ;

Orbons, S ;

Mearns, FJ ;

Shapter, JG ;

Hibbert, DB .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2003, 125 (30) :9006-9007

← 1 2 3 4 5 →