RHD epitope density profiles of RHD variant red cells analyzed by flow cytometry

被引：23

作者：

Flegel, WA ^{[1
]}

Wagner, FF ^{[1
]}

机构：

[1] UNIV ULM,ULM,GERMANY

来源：

TRANSFUSION CLINIQUE ET BIOLOGIQUE | 1996年 / 3卷 / 06期

关键词：

antigen/epitope density; D categories; flow cytometry; RHD protein variants; rhesus blood group; serology;

D O I：

10.1016/S1246-7820(96)80058-9

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

For D-II and D-IVa, RHD antigen sites per cell were tested previously only with a limited number of radio labelled anti-D. We determined RHD antigen densities (sites/cell) by flow cytometry with 64 anti-D in four partial RHD red cells. Epitope densities detected (mean +/- SD) were: D-VII 8,000 +/- 1,665 (number of anti-D used for calculation: n = 55; percentage of reference cells: 47%); DNU (D-IIlike) 6,869 +/- 1,381 (n = 47; 29%); and DHMii 20,626 +/- 4,320 (n = 55; 87%). One D-IV cell revealed two peaks with a low fluorescence range of 18,158 +/- 2,504 (n = 11; 76%) and a high range of 35,477 +/- 2,485 (n = 6; 149%). We established epitope density profiles with a large panel of anti-D for four RHD variant cells and determined the number of RHD antigens per cell.

引用

页码：429 / 431

页数：3

共 6 条

[1]

AVENT ND, 1996, TRANSF MED, V6, pS21

[2]

FLEGEL WA, 1996, TRANSFUS CLIN BIOL, V3, pS10

[3]

Flegel WA, 1996, T CLIN BIOL, V3, p33s

[4] QUANTITATION OF D SITES ON SELECTED WEAK-D AND PARTIAL-D RED-CELLS [J].