Comparison of the Rapid Methods for Screening of Group A Rotavirus in Stool Samples

被引:19
作者
Khamrin, Pattara [2 ]
Dinh Nguyen Tran [3 ]
Chan-it, Wisoot [3 ]
Thongprachum, Aksara [3 ]
Okitsu, Shoko [3 ,4 ]
Maneekarn, Niwat [2 ]
Ushijima, Hiroshi [1 ,3 ,4 ]
机构
[1] Aino Univ, Aino Hlth Sci Ctr, Shibuya Ku, Tokyo 1500002, Japan
[2] Chiang Mai Univ, Fac Med, Dept Microbiol, Chiang Mai 50000, Thailand
[3] Univ Tokyo, Grad Sch Med, Inst Int Hlth, Dept Dev Med Sci, Tokyo, Japan
[4] Aino Hlth Ctr, Aino Clin, Tokyo, Japan
基金
日本学术振兴会;
关键词
rotavirus; diarrhea; gastroenteritis; immunochromatography; Japan; IMMUNOCHROMATOGRAPHY; NOROVIRUS;
D O I
10.1093/tropej/fmq101
中图分类号
R72 [儿科学];
学科分类号
100202 ;
摘要
In this study, the sensitivity and specificity of three immunochromatography (IC) test kits for rapid detection of group A rotavirus were compared and evaluated with stool samples collected from children, who suffered from acute gastroenteritis during February to June, 2009 in Japan. A total of 86 stool samples were tested and compared with a reference RT-PCR method. The sensitivity among IP-Rota V, Dipstick Eiken ROTA and ROTA-ADENO test kits were 97.2, 95.8 and 88.7%, while the specificity were 100, 93.3 and 100%, respectively. It was demonstrated that the IC kits evaluated in this study could be used as an alternative method for the rapid screening of group A rotavirus in fecal specimens, especially during acute gastroenteritis outbreak season.
引用
收藏
页码:375 / 377
页数:3
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