Revisiting Viral RNA-Dependent RNA Polymerases: Insights from Recent Structural Studies

被引：9

作者：

Ramaswamy, Kavitha ^{[1
]}

Rashid, Mariya ^{[2
,3
]}

Ramasamy, Selvarajan ^{[4
]}

Jayavelu, Tamilselvan ^{[1
]}

Venkataraman, Sangita ^{[1
]}

机构：

[1] Anna Univ, Dept Biotechnol, Sardar Patel Rd, Chennai 600025, Tamil Nadu, India

[2] Natl Def Med Ctr, Taiwan Int Grad Program, Mol Cell Biol, Taipei 115, Taiwan

[3] Acad Sinica, Taipei 115, Taiwan

[4] Natl Res Ctr Banana, Somarasempettai Thogaimalai Rd, Podavur 639103, Tamil Nadu, India

来源：

VIRUSES-BASEL | 2022年 / 14卷 / 10期

关键词：

replication; polymerase; cofactors; structure; RNA viruses; inhibitors; SARS-COV-2; REPLICATION; INFLUENZA POLYMERASE; COMPLEX REVEALS; L PROTEIN; TRANSCRIPTION; VIRUS; MECHANISM; ACTIVATION; BINDING; SURFACE;

D O I：

10.3390/v14102200

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

RNA-dependent RNA polymerases (RdRPs) represent a distinctive yet versatile class of nucleic acid polymerases encoded by RNA viruses for the replication and transcription of their genome. The structure of the RdRP is comparable to that of a cupped right hand consisting of fingers, palm, and thumb subdomains. Despite the presence of a common structural core, the RdRPs differ significantly in the mechanistic details of RNA binding and polymerization. The present review aims at exploring these incongruities in light of recent structural studies of RdRP complexes with diverse cofactors, RNA moieties, analogs, and inhibitors.

引用

页数：39

共 98 条

[1] Structure of a paramyxovirus polymerase complex reveals a unique methyltransferase-CTD conformation [J].

Abdella, Ryan ;

Aggarwal, Megha ;

Okura, Takashi ;

Lamb, Robert A. ;

He, Yuan .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2020, 117 (09) :4931-4941

[2]

Amroun A, 2017, CRIT REV MICROBIOL, V43, P753, DOI [10.1080/1040841X.2017.1307805, 10.1080/1040841x.2017.1307805]

[3] Two RNA Tunnel Inhibitors Bind in Highly Conserved Sites in Dengue Virus NS5 Polymerase: Structural and Functional Studies [J].

Arora, Rishi ;

Liew, Chong Wai ;

Soh, Tingjin Sherryl ;

Otoo, Dorcas Adobea ;

Seh, Cheah Chen ;

Yue, Kimberley ;

Nilar, Shahul ;

Wang, Gang ;

Yokokawa, Fumiaki ;

Noble, Christian G. ;

Chen, Yen Liang ;

Shi, Pei-Yong ;

Lescar, Julien ;

Smith, Thomas M. ;

Benson, Timothy E. ;

Lim, Siew Pheng .

JOURNAL OF VIROLOGY, 2020, 94 (24)

[4] Structural snapshots of La Crosse virus polymerase reveal the mechanisms underlying Peribunyaviridae replication and transcription [J].

Arragain, Benoit ;

Trouilleton, Quentin Durieux ;

Baudin, Florence ;

Provaznik, Jan ;

Azevedo, Nayara ;

Cusack, Stephen ;

Schoehn, Guy ;

Malet, Helene .

NATURE COMMUNICATIONS, 2022, 13 (01)

[5] Pre-initiation and elongation structures of full-length La Crosse virus polymerase reveal functionally important conformational changes [J].

Arragain, Benoit ;

Effantin, Gregory ;

Gerlach, Piotr ;

Reguera, Juan ;

Schoehn, Guy ;

Cusack, Stephen ;

Malet, Helene .

NATURE COMMUNICATIONS, 2020, 11 (01)

[6] A structural and primary sequence comparison of the viral RNA-dependent RNA polymerases [J].

Bruenn, JA .

NUCLEIC ACIDS RESEARCH, 2003, 31 (07) :1821-1829

[7] Host ANP32A mediates the assembly of the influenza virus replicase [J].

Carrique, Loic ;

Fan, Haitian ;

Walker, Alexander P. ;

Keown, Jeremy R. ;

Sharps, Jane ;

Staller, Ecco ;

Barclay, Wendy S. ;

Fodor, Ervin ;

Grimes, Jonathan M. .

NATURE, 2020, 587 (7835) :638-+

[8] Evolution of Tertiary Structure of Viral RNA Dependent Polymerases [J].

Cerny, Jiri ;

Bolfikova, Barbora Cerna ;

Valdes, James J. ;

Grubhoffer, Libor ;

Ruzek, Daniel .

PLOS ONE, 2014, 9 (05)

[9] Ensemble cryo-EM reveals conformational states of the nsp13 helicase in the SARS-CoV-2 helicase replication-transcription complex [J].

Chen, James ;

Wang, Qi ;

Malone, Brandon ;

Llewellyn, Eliza ;

Pechersky, Yakov ;

Maruthi, Kashyap ;

Eng, Ed T. ;

Perry, Jason K. ;

Campbell, Elizabeth A. ;

Shaw, David E. ;

Darst, Seth A. .

NATURE STRUCTURAL & MOLECULAR BIOLOGY, 2022, 29 (03) :250-+

[10] Structural Basis for Helicase-Polymerase Coupling in the SARS-CoV-2 Replication-Transcription Complex [J].

Chen, James ;

Malone, Brandon ;

Llewellyn, Eliza ;

Grasso, Michael ;

Shelton, Patrick M. M. ;

Olinares, Paul Dominic B. ;

Maruthi, Kashyap ;

Eng, Edward T. ;

Vatandaslar, Hasan ;

Chait, Brian T. ;

Kapoor, Tarun M. ;

Darst, Seth A. ;

Campbell, Elizabeth A. .

CELL, 2020, 182 (06) :1560-+

← 1 2 3 4 5 6 7 8 9 10 →