Toxoplasma gondii Tyrosine-Rich Oocyst Wall Protein: A Closer Look through an In Silico Prism

被引:5
作者
Asghari, Ali [1 ]
Majidiani, Hamidreza [2 ]
Nemati, Taher [3 ]
Fatollahzadeh, Mohammad [3 ]
Shams, Morteza [2 ]
Naserifar, Razi [2 ]
Kordi, Bahareh [4 ]
机构
[1] Shiraz Univ Med Sci, Sch Med, Dept Med Parasitol & Mycol, Shiraz, Iran
[2] Ilam Univ Med Sci, Zoonot Dis Res Ctr, Ilam, Iran
[3] Isfahan Univ Med Sci, Sch Med, Dept Parasitol & Mycol, Esfahan, Iran
[4] Neyshabur Univ Med Sci, Dept Basic Med Sci, Neyshabur, Iran
关键词
B-CELL EPITOPES; PREDICTION; EIMERIA; SEROPREVALENCE; SURVIVAL; SEQUENCE; DESIGN;
D O I
10.1155/2021/1315618
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Toxoplasmosis is a global threat with significant zoonotic concern. The present in silico study was aimed at determination of bioinformatics features and immunogenic epitopes of a tyrosine-rich oocyst wall protein (TrOWP) of Toxoplasma gondii. After retrieving the amino acid sequence from UniProt database, several parameters were predicted including antigenicity, allergenicity, solubility and physico-chemical features, signal peptide, transmembrane domain, and posttranslational modifications. Following secondary and tertiary structure prediction, the 3D model was refined, and immunogenic epitopes were forecasted. It was a 25.57 kDa hydrophilic molecule with 236 residues, a signal peptide, and significant antigenicity scores. Moreover, several linear and conformational B-cell epitopes were present. Also, potential mouse and human cytotoxic T-lymphocyte (CTL) and helper T-lymphocyte (HTL) epitopes were predicted in the sequence. The findings of the present in silico study are promising as they render beneficial characteristics of TrOWP to be included in future vaccination experiments.
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页数:13
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