A gold immunochromatographic assay for simultaneous detection of parathion and triazophos in agricultural products

被引：34

作者：

Liu, Beibei ^{[1
]}

Gong, Hang ^{[1
,2
]}

Wang, Yulong ^{[1
]}

Zhang, Xiaoshuai ^{[3
]}

Li, Pan ^{[1
]}

Qiu, Yulou ^{[1
]}

Wang, Limin ^{[4
]}

Hua, Xiude ^{[4
]}

Guo, Yirong ^{[5
]}

Wang, Minghua ^{[4
]}

Liu, Fengquan ^{[4
]}

Liu, Xianjin ^{[1
]}

Zhang, Cunzheng ^{[1
]}

机构：

[1] Jiangsu Acad Agr Sci, Key Lab Food Qual & Safety Jiangsu Prov, Key Lab Control Technol & Stand Agroprod Safety &, State Key Lab Breeding Base,Minist Agr,Inst Food, Nanjing 210014, Jiangsu, Peoples R China

[2] Anhui Sci & Technol Univ, Coll Food & Drug, Fengyang 233100, Peoples R China

[3] Lanzhou Univ, Sch Pharm, Lanzhou 730000, Gansu, Peoples R China

[4] Nanjing Agr Univ, Coll Plant Protect, Nanjing 210095, Jiangsu, Peoples R China

[5] Zhejiang Univ, Inst Pesticide & Environm Toxicol, Hangzhou 310058, Zhejiang, Peoples R China

来源：

ANALYTICAL METHODS | 2018年 / 10卷 / 04期

关键词：

ENZYME-LINKED-IMMUNOSORBENT; MASS-SPECTROMETRY; RAPID DETECTION; AFLATOXIN B-1; SAMPLES; STRIP; ZEARALENONE; PESTICIDES; RESIDUES; FOOD;

D O I：

10.1039/c7ay02481a

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A multi-component gold immunochromatographic assay (GICA) was developed for the rapid and simultaneous detection of parathion and triazophos in agricultural products. Two different antigens were immobilized separately in two test lines on a nitrocellulose membrane, and two specific monoclonal antibodies (McAbs) against parathion and triazophos were labeled with colloidal gold nanoparticles (AuNPs) as detection probes, respectively. After optimization, the visual cut-off limits of detection for the strip test were 0.05 mu g mL(-1) for triazophos and 0.1 mu g mL(-1) for parathion. Thereafter, the developed GICA strip was employed and evaluated for the real sample test using spiked agricultural products (rice, vegetable and fruit samples). The results showed that matrix interference was negligible and the qualification was in good agreement with the results of gas chromatography (GC). The rapid, simple and low cost multidetection sensor could be a potential alternative format for on-site and rapid simultaneous detection of parathion and triazophos.

引用

页码：422 / 428

页数：7

共 34 条

[1] A sensitive chemiluminescence enzyme immunoassay based on molecularly imprinted polymers solid-phase extraction of parathion [J].