Expressed Protein Selenoester Ligation

被引：23

作者：

Kulkarni, Sameer S. ^{[1
,2
]}

Watson, Emma E. ^{[1
,2
]}

Maxwell, Joshua W. C. ^{[1
,2
]}

Niederacher, Gerhard ^{[3
]}

Johansen-Leete, Jason ^{[1
,2
]}

Huhmann, Susanne ^{[3
]}

Mukherjee, Somnath ^{[3
]}

Norman, Alexander R. ^{[1
,2
]}

Kriegesmann, Julia ^{[3
]}

Becker, Christian F. W. ^{[3
]}

Payne, Richard J. ^{[1
,2
]}

机构：

[1] Univ Sydney, Sch Chem, Ctr Excellence Innovat Peptide & Prot Sci, Sydney, NSW 2006, Australia

[2] Univ Sydney, Australian Res Council, Ctr Excellence Innovat Peptide & Prot Sci, Sydney, NSW 2006, Australia

[3] Univ Vienna, Fac Chem, Inst Biol Chem, Vienna, Austria

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2022年 / 61卷 / 20期

基金：

奥地利科学基金会; 澳大利亚研究理事会;

关键词：

Expressed Protein Selenoesters; Peptides; Protein Modifications; Protein Semi-Synthesis; Proteins; TOTAL CHEMICAL-SYNTHESIS; SELENOCYSTEINE; PEPTIDE; CYSTEINE; SEMISYNTHESIS; POLYMERASE; HYDRAZIDES;

D O I：

10.1002/anie.202200163

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Herein, we describe the development and application of a novel expressed protein selenoester ligation (EPSL) methodology for the one-pot semi-synthesis of modified proteins. EPSL harnesses the rapid kinetics of ligation reactions between modified synthetic selenopeptides and protein aryl selenoesters (generated from expressed intein fusion precursors) followed by in situ chemoselective deselenization to afford target proteins at concentrations that preclude the use of traditional ligation methods. The utility of the EPSL technology is showcased through the efficient semi-synthesis of ubiquitinated polypeptides, lipidated analogues of the membrane-associated GTPase YPT6, and site-specifically phosphorylated variants of the oligomeric chaperone protein Hsp27 at high dilution.

引用

页数：6

共 70 条

[1] Native Chemical Ligation and Extended Methods: Mechanisms, Catalysis, Scope, and Limitations [J].

Agouridas, Vangelis ;

El Mahdi, Ouafaa ;

Diemer, Vincent ;

Cargoet, Marine ;

Monbaliu, Jean-Christophe M. ;

Melnyk, Oleg .

CHEMICAL REVIEWS, 2019, 119 (12) :7328-7443

[2] UbiSite approach for comprehensive mapping of lysine and N-terminal ubiquitination sites [J].

Akimov, Vyacheslav ;

Barrio-Hernandez, Inigo ;

Hansen, Sten V. F. ;

Hallenborg, Philip ;

Pedersen, Anna-Kathrine ;

Bekker-Jensen, Dorte B. ;

Puglia, Michele ;

Christensen, Stine D. K. ;

Vanselow, Jens T. ;

Nielsen, Mogens M. ;

Kratchmarova, Irina ;

Kelstrup, Christian D. ;

Olsen, Jesper, V ;

Blagoev, Blagoy .

NATURE STRUCTURAL & MOLECULAR BIOLOGY, 2018, 25 (07) :631-+

[3]

[Anonymous], 2007, ANGEW CHEM-GER EDIT, V119, P9408

[4]

[Anonymous], 2000, ANGEW CHEM, V112, P458

[5]

[Anonymous], 2011, ANGEW CHEM-GER EDIT, V123, P7787

[6]

[Anonymous], 2011, ANGEW CHEM, V123, P6261

[7]

[Anonymous], 2008, ANGEW CHEM, V120, P10182

[8]

[Anonymous], 2016, ANGEW CHEM-GER EDIT, V128, P11569

[9]

[Anonymous], 2010, ANGEW CHEM, V122, P9607

[10]

[Anonymous], 2015, ANGEW CHEM-GER EDIT, V127, P12907

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