Comparative proteomic analysis of the heterosis phenomenon in papaya roots

被引:8
作者
Vale, Ellen Moura [1 ,2 ]
Reis, Ricardo Souza [1 ,2 ]
Santa-Catarina, Renato [3 ]
Pereira, Messias Gonzaga [3 ]
Santa-Catarina, Claudete [4 ]
Silveira, Vanildo [1 ,2 ]
机构
[1] Univ Estadual Norte Fluminense Darcy Ribeiro UENF, Ctr Biociencias & Biotecnol CBB, Lab Biotecnol, Av Alberto Lamego 2000, BR-28013602 Campos Dos Goytacazes, RJ, Brazil
[2] Univ Estadual N Fluminen, Setor Genom & Prote, Unidade Biol Integrat, Av Alberto Lamego 2000, BR-28013602 Campos dos Goytacazes, RJ, Brazil
[3] Ctr Ciencias & Tecnol Agr CCTA UENF, Lab Melhoramento & Genet Vegetal, Campos Dos Goytacazes, RJ, Brazil
[4] CBB UENF, Lab Biol Celular & Tecidual, Campos Dos Goytacazes, RJ, Brazil
关键词
Heterosis; Hybrid vigor; Label free; Proteome; Papaya roots; HDMSE; KINASE KINASE KINASE; PROTEIN-KINASE; DIRIGENT PROTEINS; COMBINING ABILITY; AUXIN TRANSPORT; LATERAL ROOT; ARABIDOPSIS; TOLERANCE; HYBRID; GENES;
D O I
10.1016/j.scienta.2016.06.031
中图分类号
S6 [园艺];
学科分类号
0902 ;
摘要
Heterosis describes the superior performance of a heterozygous F-1-hybrid in comparison withthe average performance of the parental lines for a given trait in a given environment and is the result of the effects of non-additive genes. In-the present study, proteins from the primary roots of the papaya (Carica papaya L.) hybrid JS12 x Sao Mateus and its parental inbred lines were analyzed using proteomic analyses combining the shotgun method and nanoESI-HDMSE technology. A total of 955 proteins were identified by the shotgun method, among which 261 exhibited a trend toward heterosis in the hybrid compared with the mid-parents. Non-additive proteins were divided into "above high-parent" (16.1%), "high-parent" (6.5%), "low-parent" (22.2%), and "below low-parent" (55.2%) abundance patterns. The results revealed a decrease in proteins involved in energy-consuming processes such as protein metabolism and an increase in-root development proteins such as those involved in auxin polar transport and signaling regulation. The findings suggest that the hybrid possesses an optimization mechanism for protein synthesis that results in substantial improvements in cellular energy efficiency and phenotypic performance. Therefore, this study may contribute to a better understanding of the molecular basis of heterosis in papaya. (C) 2016 Elsevier B.V. All rights reserved.
引用
收藏
页码:178 / 186
页数:9
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