Matrix-Free DNP-Enhanced NMR Spectroscopy of Liposomes Using a Lipid-Anchored Biradical

被引：50

作者：

Fernandez-de-Alba, Carlos ^{[1
,2
]}

Takahashi, Hiroki ^{[1
,2
]}

Richard, Alexandre ^{[1
,2
]}

Chenavier, Yves ^{[1
,2
]}

Dubois, Lionel ^{[1
,2
]}

Maurel, Vincent ^{[1
,2
]}

Lee, Daniel ^{[1
,2
]}

Hediger, Sabine ^{[1
,2
,3
]}

De Paepe, Gael ^{[1
,2
]}

机构：

[1] Univ Grenoble Alpes, INAC, LCIB, F-38000 Grenoble, France

[2] CEA Grenoble, INAC, LCIB, F-38054 Grenoble, France

[3] CNRS, INAC, LCIB, F-38000 Grenoble, France

来源：

CHEMISTRY-A EUROPEAN JOURNAL | 2015年 / 21卷 / 12期

关键词：

dynamic nuclear polarization; membranes; phospholipids; NMR spectroscopy; solid-state NMR spectroscopy; DYNAMIC NUCLEAR-POLARIZATION; SOLID-STATE NMR; C-13-C-13 CORRELATION SPECTROSCOPY; INTERDIGITATED GEL PHASE; SIGNAL ENHANCEMENT; MEMBRANES; PROTEINS;

D O I：

10.1002/chem.201404588

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Magic-angle spinning dynamic nuclear polarization (MAS-DNP) has been proven to be a powerful technique to enhance the sensitivity of solid-state NMR (SSNMR) in a wide range of systems. Here, we show that DNP can be used to polarize lipids using a lipid-anchored polarizing agent. More specifically, we introduce a C16-functionalized biradical, which allows localization of the polarizing agents in the lipid bilayer and DNP experiments to be performed in the absence of excess cryo-protectant molecules (glycerol, dimethyl sulfoxide, etc.). This constitutes another original example of the matrix-free DNP approach that we recently introduced.

引用

页码：4512 / +

页数：6

共 37 条

[1]

Aisenbrey Christopher, 2013, Methods Mol Biol, V974, P357, DOI 10.1007/978-1-62703-275-9_16

[2] INSIGHTS INTO THE CRYOPROTECTIVE MECHANISM OF DIMETHYL-SULFOXIDE FOR PHOSPHOLIPID-BILAYERS [J].

ANCHORDOGUY, TJ ;

CECCHINI, CA ;

CROWE, JH ;

CROWE, LM .

CRYOBIOLOGY, 1991, 28 (05) :467-473

[3] Dynamic Nuclear Polarization Study of Inhibitor Binding to the M218-60 Proton Transporter from Influenza A [J].

Andreas, Loren B. ;

Barnes, Alexander B. ;

Corzilius, Bjoern ;

Chou, James J. ;

Miller, Eric A. ;

Caporini, Marc ;

Rosay, Melanie ;

Griffin, Robert G. .

BIOCHEMISTRY, 2013, 52 (16) :2774-2782

[4] Influence of lipids on ice formation in cryoprotective media [J].

Andreev A.A. ;

Sadikova D.G. ;

Labbe C. ;

Ananiev V.I. ;

Kurchikov A.L. .

Biophysics, 2008, 53 (4) :283-285

[5]

Baldus M., 2013, 8 ALP C SOL STAT NMR

[6] DIFFUSION OF UNIVALENT IONS ACROSS LAMELLAE OF SWOLLEN PHOSPHOLIPIDS [J].

BANGHAM, AD ;

STANDISH, MM ;

WATKINS, JC .

JOURNAL OF MOLECULAR BIOLOGY, 1965, 13 (01) :238-+

[7] ACTION OF STEROIDS AND STREPTOLYSIN S ON PERMEABILITY OF PHOSPHOLIPID STRUCTURES TO CATIONS [J].

BANGHAM, AD ;

STANDISH, MM ;

WEISSMANN, G .

JOURNAL OF MOLECULAR BIOLOGY, 1965, 13 (01) :253-+

[8] EFFECT OF GLYCEROL ON THE INTERFACIAL PROPERTIES OF DIPALMITOYLPHOSPHATIDYLCHOLINE LIPOSOMES AS MEASURED WITH MEROCYANINE-540 [J].

BIONDI, AC ;

DISALVO, EA .

BIOCHIMICA ET BIOPHYSICA ACTA, 1990, 1028 (01) :43-48

[9] INTERDIGITATED GEL PHASE BILAYERS FORMED BY UNSATURATED SYNTHETIC AND BACTERIAL GLYCEROLIPIDS IN THE PRESENCE OF POLYMYXIN-B AND GLYCEROL [J].

BOGGS, JM ;

TUMMLER, B .

BIOCHIMICA ET BIOPHYSICA ACTA, 1993, 1145 (01) :42-50

[10]

Crowe J.H., 2011, HDB PHYSL PHYSL, P1445, DOI [DOI 10.1002/CPHY.CP130220, 10.1002/cphy.cp130220]

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