Modified Microtiter count method for viable cell counts from pure cultures and food model samples

被引：9

作者：

Kim, S ^{[1
]}

Fung, DYC ^{[1
]}

机构：

[1] Kansas State Univ, Food Microbiol Lab, Dept Anim Sci & Ind, Manhattan, KS 66506 USA

来源：

FOOD MICROBIOLOGY | 2005年 / 22卷 / 06期

关键词：

microtiter count; spiral plate; viable cell counts;

D O I：

10.1016/j.fm.2004.11.011

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A modified Microtiter count method was designed to assess viable cell counts in a rapid, easy, and accurate way. A Spiral plate method was used for comparison with the modified Microtiter count method. There was a strong correlation between the two methods in microbial counts from pure cultures (Escherichia coli O157:H7, R-2 = 0.984; Salmonella enterica serovar Enteritidis, R-2 = 0.995; and Listeria monocytogenes, R-2 = 0.994) and food samples (R-2 = 0.897). This study suggests that a modified Microtiter count method can be used to determine viable bacterial cell counts. (c) 2005 Elsevier Ltd. All rights reserved.

引用

页码：595 / 599

页数：5

共 19 条

[1]

BALDOCK JD, 1968, J APPL MICROBIOL, V16, P1627

[2] MICROTITER TECHNIQUE FOR ENUMERATION OF MESOPHILES, PSYCHROTROPHS, AND COLIFORMS IN RAW AND PASTEURIZED MILK [J].

CASAS, IA ;

LEON, N ;

IZQUIERDO, P .

JOURNAL OF FOOD PROTECTION, 1977, 40 (11) :795-797

[3] COMPARISON OF REDIGEL, PETRIFILM, SPIRAL PLATE SYSTEM, ISOGRID, AND AEROBIC PLATE-COUNT FOR DETERMINING THE NUMBERS OF AEROBIC-BACTERIA IN SELECTED FOODS [J].

CHAIN, VS ;

FUNG, DYC .

JOURNAL OF FOOD PROTECTION, 1991, 54 (03) :208-211

[4]

CHEN RK, 1976, ABS ANN M AM SOC MIC, P33

[5]

Fung D. Y. C., 1995, J RAPID METH AUT MIC, V3, P237

[6] COLLABORATIVE STUDY OF MICROTITER COUNT METHOD AND STANDARD PLATE COUNT METHOD FOR VIABLE CELL COUNT OF RAW MILK [J].

FUNG, DYC ;

DONAHUE, R ;

JENSEN, JP ;

ULLMANN, WW ;

HAUSLER, WJ ;

LAGRANGE, W .

JOURNAL OF MILK AND FOOD TECHNOLOGY, 1976, 39 (01) :24-26

[7] MINIATURIZED TECHNIQUES FOR IMVIC TESTS [J].

FUNG, DYC ;

MILLER, RD .

JOURNAL OF MILK AND FOOD TECHNOLOGY, 1972, 35 (06) :328-&

[8] COMPARATIVE-ANALYSIS OF REDIGEL AND AEROBIC PLATE-COUNT METHODS FOR VIABLE CELL COUNTS OF SELECTED FOODS [J].

FUNG, DYC ;

CHAIN, VS .

FOOD MICROBIOLOGY, 1991, 8 (04) :299-301

[9] EFFECT OF DYES ON BACTERIAL GROWTH [J].

FUNG, DYC ;

MILLER, RD .

APPLIED MICROBIOLOGY, 1973, 25 (05) :793-799

[10]

Fung DYC, 2000, IRISH J AGR FOOD RES, V39, P301

← 1 2 →