Modified Microtiter count method for viable cell counts from pure cultures and food model samples

被引:9
作者
Kim, S [1 ]
Fung, DYC [1 ]
机构
[1] Kansas State Univ, Food Microbiol Lab, Dept Anim Sci & Ind, Manhattan, KS 66506 USA
关键词
microtiter count; spiral plate; viable cell counts;
D O I
10.1016/j.fm.2004.11.011
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
A modified Microtiter count method was designed to assess viable cell counts in a rapid, easy, and accurate way. A Spiral plate method was used for comparison with the modified Microtiter count method. There was a strong correlation between the two methods in microbial counts from pure cultures (Escherichia coli O157:H7, R-2 = 0.984; Salmonella enterica serovar Enteritidis, R-2 = 0.995; and Listeria monocytogenes, R-2 = 0.994) and food samples (R-2 = 0.897). This study suggests that a modified Microtiter count method can be used to determine viable bacterial cell counts. (c) 2005 Elsevier Ltd. All rights reserved.
引用
收藏
页码:595 / 599
页数:5
相关论文
共 19 条