A3-Hydroxythalidomide-based Ratiometric FluorescentProbe for the Detection of H2O2

Off-label use of3-hydroxythalidomide draws more and more attentions due to its known biocompatibility???the metabolic pathway and the unique binding mode to CRBN protein.However???there is rare report about the application in analysis field.Herein???the off-label use of3-hydroxythalidomide in the field of chemosensor was investi???gated.We designed and synthesized a H2O2probe2-???2???6-dioxopiperidin-3-yl???-4-??????4-???4???4???5???5-tetramethyl-1???3???2-dioxaborolan-2-yl???-benzyl???oxy???isoindoline-1???3-dione with fluorescent properties???using the old drug3-hydroxythali-domide as parent compound.Based on the analysis???the probe has high selectivity and sensitivity for H2O2over otheranalysts based on the exited state intramolecular proton transfer mechanism.Furthermore???the probe has goodselectivity towards other analysts with a limit of detection???LOD???of9.8nmol/L???and it was successfully used in the de???tection of H2O2in cosmetics samples???water samples and sewage samples.Meanwhile???we revealed the sensoringmechanism through the spectrum technology and the theoretic calculation