Advances in molecular-based diagnostics in meeting crop biosecurity and phytosanitary issues

被引:137
作者
Schaad, NW [1 ]
Frederick, RD
Shaw, J
Schneider, WL
Hickson, R
Petrillo, MD
Luster, DG
机构
[1] USDA, ARS, Foreign Dis Weed Sci Res Unit, Ft Detrick, MD 21702 USA
[2] Lexicon Genet, The Woodlands, TX 77381 USA
[3] Joint Special Operat Univ, Hulburt Field, FL 32544 USA
[4] USDA, APHIS, San Francisco, CA 94080 USA
关键词
polymerase chain reaction (PCR); real-time PCR; bacteria; viruses; fungi; and nematodes;
D O I
10.1146/annurev.phyto.41.052002.095435
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Awareness of crop biosecurity and phytosanitation has been heightened since 9/11 and the unresolved anthrax releases in October 2001. Crops are highly vulnerable to accidental or deliberate introductions of crop pathogens from outside U.S. borders. Strategic thinking about protection against deliberate or accidental release of a plant pathogen is an urgent priority. Rapid detection will be the key to success. This review summarizes recent progress in the development of rapid real-time PCR protocols and evaluates their effectiveness in a proposed nationwide network of diagnostic laboratories that will facilitate rapid diagnostics and improved communication.
引用
收藏
页码:305 / 324
页数:24
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