Anti-Helicobacter pylori Activity of a Lactobacillus sp. PW-7 Exopolysaccharide

被引：23
作者：
Hu, Jingfei [1 ,2 ]
Tian, Xueqing [1 ,2 ]
Wei, Tong [1 ,2 ]
Wu, Hangjie [1 ,2 ]
Lu, Jing [1 ,2 ]
Lyu, Mingsheng [1 ,2 ]
Wang, Shujun [1 ,2 ]
机构：
[1] Jiangsu Ocean Univ, Jiangsu Key Lab Marine Bioresources & Environm, Jiangsu Key Lab Marine Biotechnol, Lianyungang 222005, Peoples R China
[2] Jiangsu Ocean Univ, Coinnovat Ctr Jiangsu Marine Bioind Technol, Lianyungang 222005, Peoples R China
来源：
FOODS | 2021年 / 10卷 / 10期
基金：
国家重点研发计划;
关键词：
Helicobacter pylori; Lactobacillus plajomi; exopolysaccharide; antibacterial activity; antioxidant activity; LACTIC-ACID BACTERIA; ANTIBACTERIAL ACTIVITY; ESCHERICHIA-COLI; FERMENTUM UCO-979C; PROBIOTIC STRAIN; ESSENTIAL OIL; PURIFICATION; INFECTION;
D O I：
10.3390/foods10102453
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
Helicobacter pylori is a cause of gastric cancer. We extracted the exopolysaccharide (EPS) of Lactobacillus plajomi PW-7 for antibacterial activity versus H. pylori, elucidating its biological activity and structural characteristics. The minimum inhibitory concentration (MIC) of EPS against H. pylori was 50 mg/mL. Disruption of the cell membranes of pathogenic bacteria by EPS was indicated via the antibacterial mechanism test and confirmed through electron microscopy. EPS also has antioxidant capacity. The IC50 of EPS for 2,2-Diphenyl-1-picrylhydrazyl (DPPH) radical, superoxide anions, and hydroxyl radicals were 300 mu g/mL, 180 mu g/mL, and 10 mg/mL, respectively. The reducing power of EPS was 2 mg/mL, equivalent to 20 mu g/mL of ascorbic acid. EPS is a heteropolysaccharide comprising six monosaccharides, with an approximate molecular weight of 2.33 x 10(4) Da. Xylose had a significant effect on H. pylori. EPS from L. plajomi PW-7 showed potential as an antibacterial compound and antioxidant, laying a foundation for the development of EPS-based foods.</p>
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页数：15
相关论文
共 54 条
[1]  
Abdullah A., 2021, IOP C SERIES MAT SCI, V1053
[2]   In vitro antioxidant, antibacterial, in vivo immunomodulatory, antitumor and hematological potential of exopolysaccharide produced by wild type and mutant Lactobacillus delbureckii subsp. bulgaricus [J].
Adebayo-Tayo, Bukola ;
Fashogbon, Racheal .
HELIYON, 2020, 6 (02)
[3]   Lactobacillus fermentum: Could EPS production ability be responsible for functional properties? [J].
Ale, Elisa C. ;
Rojas, Maria F. ;
Reinheimer, Jorge A. ;
Binetti, Ana G. .
FOOD MICROBIOLOGY, 2020, 90
[4]   Characterization, bioactivities, and rheological properties of exopolysaccharide produced by novel probiotic Lactobacillus plantarum C70 isolated from camel milk [J].
Ayyash, Mutamed ;
Abu-Jdayil, Basim ;
Itsaranuwat, Pariyaporn ;
Galiwango, Emmanuel ;
Tamiello-Rosa, Camila ;
Abdullah, Hassan ;
Esposito, Gennaro ;
Hunashal, Yamanappa ;
Obaid, Reyad S. ;
Hamed, Fathalla .
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2020, 144 :938-946
[5]   Genetic diversity of Lactobacillus spp. isolates from oral cavity and their probiotic and antimicrobial properties [J].
Azizian, Khalil ;
Osquee, Hamid Owaysee ;
Pourlak, Tala ;
Hosseinpour, Rasoul ;
Asgharzadeh, Mohammad ;
Ganvarov, Khudaverdi ;
Kafil, Hossein Samadi .
GENE REPORTS, 2021, 24
[6]   Partial characterization and antioxidant activity of exopolysaccharides produced by Lactobacillus plantarum CNPC003 [J].
Bomfim, Vanessa Barbosa ;
Pereira Lopes Neto, Jose Honorio ;
Leite, Kerolayne Santos ;
Vieira, Erica de Andrade ;
Iacomini, Marcello ;
Silva, Caroline Mellinger ;
Olbrich dos Santos, Karina Maria ;
Cardarelli, Haissa Roberta .
LWT-FOOD SCIENCE AND TECHNOLOGY, 2020, 127
[7]   Exopolysaccharides produced by lactic acid bacteria: from health-promoting benefits to stress tolerance mechanisms [J].
Caggianiello, Graziano ;
Kleerebezem, Michiel ;
Spano, Giuseppe .
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2016, 100 (09) :3877-3886
[8]   Screening lactic acid bacteria from pickle and cured meat as biocontrol agents of Penicillium digitatum on citrus fruit [J].
Chen, Ou ;
Hong, Yang ;
Ma, Jiahong ;
Deng, Lili ;
Yi, Lanhua ;
Zeng, Kaifang .
BIOLOGICAL CONTROL, 2021, 158
[9]   In vitro screening of lactobacilli with antagonistic activity against Helicobacter pylori from traditionally fermented foods [J].
Chen, X. ;
Tian, F. ;
Liu, X. ;
Zhao, J. ;
Zhang, H. -P. ;
Zhang, H. ;
Chen, W. .
JOURNAL OF DAIRY SCIENCE, 2010, 93 (12) :5627-5634
[10]   A food additive with prebiotic properties of an α-D-glucan from Lactobacillus plantarum DM5 [J].
Das, Deeplina ;
Baruah, Rwivoo ;
Goyal, Arun .
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2014, 69 :20-26
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