Evaluation of photodynamic therapy (PDT) procedures using microfluidic system

被引：23

作者：

Jedrych, Elzbieta ^{[1
]}

Pawlicka, Zuzanna ^{[1
]}

Chudy, Michal ^{[1
]}

Dybko, Artur ^{[1
]}

Brzozka, Zbigniew ^{[1
]}

机构：

[1] Warsaw Univ Technol, Dept Microbioanalyt, Fac Chem, PL-00664 Warsaw, Poland

来源：

ANALYTICA CHIMICA ACTA | 2011年 / 683卷 / 02期

关键词：

Photodynamic therapy (PDT); Microfluidic system; Lab on a chip; Cancer treatment; Cell culture; 5-AMINOLEVULINIC ACID; CELL-CULTURE; IN-VITRO; METHYL-ESTER; CANCER; EFFLUX;

D O I：

10.1016/j.aca.2010.10.005

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A hybrid PDMS/glass microfluidic system for evaluation of the efficiency of photodynamic therapy is presented. 5-aminolevulinic acid (ALA) was used as a precursor of photosensitizer. The geometry of the microdevice presented in this paper enables to test different concentrations of the photosensitizer in a single assay. The viability of the A549 cells was determined 24h after PDT procedure (irradiation with light which induced a photosensitizer accumulated in carcinoma cells, lambda = 625 nm). The presented results confirmed the possibility to perform the photodynamic therapy process in vitro in microscale and the possibility to assess its effectiveness. Moreover, because two identical microstructures on a single chip were performed, the microchip can be used for examination simultaneously various cell lines (carcinoma and normal) or various photosensitizers. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：149 / 155

页数：7

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